TCR V α 8 Antibody (KT50): sc-53477
- TCR V α 8 Antibody (KT50) is a rat monoclonal IgG2a TCR V α 8 antibody provided at 200 µg/ml
- raised against V alpha 8 T cell receptor of mouse origin
- TCR V alpha 8 Antibody (KT50) is recommended for detection of TCR V α 8 of mouse origin by IF and FCM
- Anti-TCR V alpha 8 Antibody (KT50) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TCR V α 8 Antibody (KT50). This work is in progress.
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TCR V α 8 Antibody (KT50) is a rat monoclonal IgG2a antibody that detects TCR V alpha 8 protein of mouse origin by immunofluorescence (IF) and flow cytometry (FCM). TCR V α 8 (KT50) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The T cell antigen receptor (TCR) plays a crucial role in immune response by recognizing diverse foreign antigens, which is essential for T cell activation and subsequent immune response. TCR is a heterodimer consisting of either alpha and beta chains or gamma and delta chains, with most T cells (approximately 95%) expressing the alpha/beta heterodimer. TCR recognition of various antigens occurs through somatic recombination, involving rearrangement of variable (V), diversity (D), and joining (J) gene segments during T cell development. This complex assembly process creates a vast repertoire of TCRs, enabling effective immune system response to numerous pathogens. In mice, TCR alpha chain is constructed from 104 V alpha segments and 61 J alpha segments located at alpha/delta loci, while human equivalent has fewer V alpha segments but similar J alpha segments. TCR specificity and diversity are vital for adaptive immune system, making TCR V α 8 (KT50) monoclonal antibody an important tool for studying T cell activation and function in mouse models.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TCR V α 8 Antibody (KT50) References:
- The role of recombination activating gene (RAG) reinduction in thymocyte development in vivo. | Yannoutsos, N., et al. 2001. J Exp Med. 194: 471-80. PMID: 11514603
- T cell receptor (TCR) alpha/delta locus enhancer identity and position are critical for the assembly of TCR delta and alpha variable region genes. | Bassing, CH., et al. 2003. Proc Natl Acad Sci U S A. 100: 2598-603. PMID: 12604775
- Regulation of TCR delta and alpha repertoires by local and long-distance control of variable gene segment chromatin structure. | Hawwari, A. and Krangel, MS. 2005. J Exp Med. 202: 467-72. PMID: 16087716
- MR1-restricted V alpha 19i mucosal-associated invariant T cells are innate T cells in the gut lamina propria that provide a rapid and diverse cytokine response. | Kawachi, I., et al. 2006. J Immunol. 176: 1618-27. PMID: 16424191
- A model for TCR gene segment use. | Warmflash, A. and Dinner, AR. 2006. J Immunol. 177: 3857-64. PMID: 16951348
- A unique T cell receptor discovered in marsupials. | Parra, ZE., et al. 2007. Proc Natl Acad Sci U S A. 104: 9776-81. PMID: 17535902
- Developmental stage-specific regulation of TCR-alpha-chain gene assembly by intrinsic features of the TEA promoter. | Huang, CY. and Sleckman, BP. 2007. J Immunol. 179: 449-54. PMID: 17579065
- Germline-encoded recognition of diverse glycolipids by natural killer T cells. | Scott-Browne, JP., et al. 2007. Nat Immunol. 8: 1105-13. PMID: 17828267
- Accessibility control of variable region gene assembly during T-cell development. | Sleckman, BP., et al. 1998. Immunol Rev. 165: 121-30. PMID: 9850857
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TCR V α 8 Antibody (KT50) | sc-53477 | 200 µg/ml | $322.00 | |||
TCR V α 8 Antibody (KT50) AC | sc-53477 AC | 500 µg/ml, 25% agarose | $424.00 | |||
TCR V α 8 Antibody (KT50) HRP | sc-53477 HRP | 200 µg/ml | $322.00 | |||
TCR V α 8 Antibody (KT50) FITC | sc-53477 FITC | 200 µg/ml | $336.00 | |||
TCR V α 8 Antibody (KT50) PE | sc-53477 PE | 200 µg/ml | $349.00 | |||
TCR V α 8 Antibody (KT50) Alexa Fluor® 488 | sc-53477 AF488 | 200 µg/ml | $364.00 | |||
TCR V α 8 Antibody (KT50) Alexa Fluor® 546 | sc-53477 AF546 | 200 µg/ml | $364.00 | |||
TCR V α 8 Antibody (KT50) Alexa Fluor® 594 | sc-53477 AF594 | 200 µg/ml | $364.00 | |||
TCR V α 8 Antibody (KT50) Alexa Fluor® 647 | sc-53477 AF647 | 200 µg/ml | $364.00 | |||
TCR V α 8 Antibody (KT50) Alexa Fluor® 680 | sc-53477 AF680 | 200 µg/ml | $364.00 | |||
TCR V α 8 Antibody (KT50) Alexa Fluor® 790 | sc-53477 AF790 | 200 µg/ml | $364.00 |