TCF7L2/TCF4 Double Nickase Plasmid (h): sc-400607-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TCF7L2/TCF4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TCF7L2/TCF4 Double Nickase Plasmid (h) and TCF7L2/TCF4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TCF7L2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TCF7L2/TCF4 Antibody (D-4): sc-166699
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TCF7L2/TCF4 Double Nickase Plasmid (h) | sc-400607-NIC | 20 µg | $410.00 | |||
TCF7L2/TCF4 Double Nickase Plasmid (h2) | sc-400607-NIC-2 | 20 µg | $410.00 |
TCF7L2 (also known as TCF4) encodes a high-mobility group (HMG) box transcription factor that functions as a key nuclear effector of canonical Wnt/β-catenin signaling. By partnering with β-catenin and co-regulators, TCF7L2 modulates transcriptional programs controlling cell fate decisions, proliferation, and differentiation across multiple tissue contexts, including intestinal epithelium and endocrine pancreas. TCF7L2-dependent regulation influences chromatin accessibility and integrates signals from developmental and metabolic pathways to shape context-specific gene expression. Genetic variation and dysregulated activity of TCF7L2 have been linked to metabolic traits and type 2 diabetes risk, and aberrant Wnt/TCF transcriptional output is frequently studied in cancer and stem cell biology.
TCF7L2/TCF4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TCF7L2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TCF7L2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TCF7L2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TCF7L2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.