Anti-TBC1D3/3B/3C/3G Antibody (H-7) recommended for detection of TBC1D3, TBC1D3B, TBC1D3C and TBC1D3G of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-TBC1D3/3B/3C/3G Antibody (H-7): sc-376073

Anti-TBC1D3/3B/3C/3G Antibody (H-7) is rated 4.3 out of 5 by 3.
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Datasheets

    • Anti-TBC1D3/3B/3C/3G Antibody (H-7) is a mouse monoclonal IgG1 κ TBC1D3/3B/3C/3G antibody, cited in 2 publications, provided at 200 µg/ml
    • raised against amino acids 496-549 mapping at the C-terminus of TBC1D3 of human origin
    • TBC1D3/3B/3C/3G Antibody (H-7) is recommended for detection of TBC1D3, TBC1D3B, TBC1D3C and TBC1D3G of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-TBC1D3/3B/3C/3G Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of TBC1D3/3B/3C/3G (H-7): sc-376073.
    • m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for TBC1D3/3B/3C/3G Antibody (H-7) for WB and IHC(P) applications. These reagents are now offered in bundles with TBC1D3/3B/3C/3G Antibody (H-7) (see ordering information below).
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I am using TBC1D3/3B/3C/3G (H-7): sc-376073 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Randy McDonald
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from WB: robust band in human platelet extract WB: robust band in human platelet extract. -SCBT QC
Date published: 2015-06-09
Rated 3 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB. -SCBT QC
Date published: 2015-05-30
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmic Produced beautiful immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2015-03-19
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