TAT Antibody (A-6): sc-374065

TAT Antibody (A-6) is a mouse monoclonal IgG2b antibody that detects TAT (tyrosine aminotransferase) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TAT is a 454 amino acid protein that plays a crucial role in the mitochondria, where TAT functions as a homodimer and utilizes pyridoxal phosphate as a cofactor to catalyze the conversion of L-tyrosine into p-hydroxyphenylpyruvate, a key step in amino acid degradation. This enzymatic activity is vital for maintaining amino acid homeostasis and preventing the accumulation of toxic metabolites. Defects in the gene encoding TAT can lead to tyrosinemia type 2 (TYRO2), a metabolic disorder characterized by elevated levels of tyrosine in the blood and urine, which can result in serious health issues such as palmoplantar keratosis, painful corneal ulcers, and cognitive impairments. The gene encoding TAT is located on human chromosome 16, a region that encompasses over 900 genes and accounts for nearly 3% of the human genome, highlighting TAT′s importance in metabolic processes and genetic disorders. Anti-TAT antibody (A-6) serves as an essential tool for researchers studying TAT′s role in various biological contexts and metabolic diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

TAT Antibody (A-6) References:

1. Transformation of cultured fetal rat liver cells by MDAB and phenobarbital. Morphological, biochemical and immunocytochemical characterization of cell lines.  |  Yeoh, G., et al. 1989. Carcinogenesis. 10: 1015-27. PMID: 2470526
2. Hormonal regulation of TSE1-repressed genes: evidence for multiple genetic controls in extinction.  |  Thayer, MJ. and Fournier, RE. 1989. Mol Cell Biol. 9: 2837-46. PMID: 2571076
3. Regulation of tyrosine aminotransferase in rat liver. XI. Studies on the relationship of enzyme stability to enzyme turnover in cultured hepatoma cells.  |  Johnson, RW. and Kenney, FT. 1973. J Biol Chem. 248: 4528-31. PMID: 4146264
4. Dexamethasone phosphate-induced synthesis of tyrosine aminotransferase in hepatoma tissue culture cells. Studies of the early phases of induction and of the steroid requirement for maintanance of the induced rate of synthesis.  |  Granner, DK., et al. 1970. J Biol Chem. 245: 1472-8. PMID: 4392629
5. Stimulation of tyrosine aminotransferase synthesis by dexamethasone phosphate in cell culture.  |  Granner, DK., et al. 1968. J Mol Biol. 35: 291-301. PMID: 4398833
6. Induction of tyrosine aminotransferase by dexamethasone, insulin, and serum. Characterization of the induced enzyme.  |  Gelehrter, TD., et al. 1972. J Biol Chem. 247: 6197-203. PMID: 4405601
7. Analysis of insulin action using differentiated and dedifferentiated hepatoma cells.  |  Crettaz, M. and Kahn, CR. 1983. Endocrinology. 113: 1201-9. PMID: 6137358
8. Insulin regulation of insulin-like growth factor action in rat hepatoma cells.  |  Heaton, JH., et al. 1984. J Biol Chem. 259: 2396-402. PMID: 6142041
9. Insulin regulation of Na/K pump activity in rat hepatoma cells.  |  Gelehrter, TD., et al. 1984. Diabetes. 33: 428-34. PMID: 6327435
10. Differential binding of mutant glucocorticoid receptors to the glucocorticoid response element of the tyrosine aminotransferase gene.  |  Rowan, BG. and Ip, MM. 1996. J Steroid Biochem Mol Biol. 58: 147-62. PMID: 8809196

The preferred TAT antibody is TAT Antibody (H-9): sc-376292.