Tastin Antibody (D-2): sc-271715
- Tastin Antibody (D-2) is a mouse monoclonal IgG2a κ Tastin antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 73-107 within an internal region of Tastin of human origin
- Tastin Antibody (D-2) is recommended for detection of Tastin of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Tastin Antibody (D-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP is the preferred secondary detection reagent for Tastin Antibody (D-2) for WB applications. This reagent is now offered in a bundle with Tastin Antibody (D-2) (see ordering information below).
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Tastin Antibody (D-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects Tastin protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Tastin antibody (D-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tastin protein, also known as trophinin associated protein (TROAP), plays a critical role in maintaining centrosome integrity and ensuring proper bipolar organization of the mitotic spindle during cell division. Tastin (TROAP) is predominantly expressed in tissues such as bone marrow, testis, and thymus, where Tastin (TROAP) localizes to the cytoplasm and associates with microtubules, the mitotic spindle, and centrosomes. Notably, Tastin expression peaks during the G2/M phase of the cell cycle and diminishes post-division, highlighting Tastin′s importance in cell proliferation. Cells that overexpress Tastin tend to form monopolar spindles, while those with depleted levels exhibit multipolar spindles, indicating Tastin′s essential function in spindle assembly. Furthermore, Tastin interacts directly with Dynein light chain (Dynein LC or Tctex-1) and γ Tubulin, reinforcing Tastin′s role in spindle dynamics. Additionally, Tastin forms complexes with Bystin and Trophinin, which are crucial for cell adhesion and embryo implantation, underscoring Tastin′s significance in developmental processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Tastin Antibody (D-2) References:
- Expression of trophinin, tastin, and bystin by trophoblast and endometrial cells in human placenta. | Suzuki, N., et al. 1999. Biol Reprod. 60: 621-7. PMID: 10026108
- Trophinin, tastin, and bystin: a complex mediating unique attachment between trophoblastic and endometrial epithelial cells at their respective apical cell membranes. | Fukuda, MN. and Nozawa, S. 1999. Semin Reprod Endocrinol. 17: 229-34. PMID: 10797941
- Human tastin, a proline-rich cytoplasmic protein, associates with the microtubular cytoskeleton. | Nadano, D., et al. 2002. Biochem J. 364: 669-77. PMID: 12049630
- Implantation-dependent expression of trophinin by maternal fallopian tube epithelia during tubal pregnancies: possible role of human chorionic gonadotrophin on ectopic pregnancy. | Nakayama, J., et al. 2003. Am J Pathol. 163: 2211-9. PMID: 14633596
- Bystin as a novel marker for reactive astrocytes in the adult rat brain following injury. | Sheng, J., et al. 2004. Eur J Neurosci. 20: 873-84. PMID: 15305856
- Bystin in perineural invasion of prostate cancer. | Ayala, GE., et al. 2006. Prostate. 66: 266-72. PMID: 16245277
- Tastin is required for bipolar spindle assembly and centrosome integrity during mitosis. | Yang, S., et al. 2008. FASEB J. 22: 1960-72. PMID: 18218922
- Trophinin and tastin, a novel cell adhesion molecule complex with potential involvement in embryo implantation. | Fukuda, MN., et al. 1995. Genes Dev. 9: 1199-210. PMID: 7758945
- Molecular basis of embryo implantation. | Fukuda, MN. 1996. Keio J Med. 45: 37-43. PMID: 8882466
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tastin Antibody (D-2) | sc-271715 | 200 µg/ml | $322.00 | |||
Tastin Antibody (D-2): m-IgG2a BP-HRP Bundle | sc-545933 | 200 µg Ab; 10 µg BP | $361.00 | |||
Tastin Antibody (D-2) AC | sc-271715 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Tastin Antibody (D-2) HRP | sc-271715 HRP | 200 µg/ml | $322.00 | |||
Tastin Antibody (D-2) FITC | sc-271715 FITC | 200 µg/ml | $336.00 | |||
Tastin Antibody (D-2) PE | sc-271715 PE | 200 µg/ml | $349.00 | |||
Tastin Antibody (D-2) Alexa Fluor® 488 | sc-271715 AF488 | 200 µg/ml | $364.00 | |||
Tastin Antibody (D-2) Alexa Fluor® 546 | sc-271715 AF546 | 200 µg/ml | $364.00 | |||
Tastin Antibody (D-2) Alexa Fluor® 594 | sc-271715 AF594 | 200 µg/ml | $364.00 | |||
Tastin Antibody (D-2) Alexa Fluor® 647 | sc-271715 AF647 | 200 µg/ml | $364.00 | |||
Tastin Antibody (D-2) Alexa Fluor® 680 | sc-271715 AF680 | 200 µg/ml | $364.00 | |||
Tastin Antibody (D-2) Alexa Fluor® 790 | sc-271715 AF790 | 200 µg/ml | $364.00 | |||
Tastin (D-2) Neutralizing Peptide | sc-271715 P | 100 µg/0.5 ml | $69.00 |