Taspase 1 Antibody (D-6): sc-514677
- Taspase 1 Antibody (D-6) is a mouse monoclonal IgG1 κ Taspase 1 antibody provided at 200 µg/ml
- raised against amino acids 289-420 mapping at the C-terminus of Taspase 1 of human origin
- Taspase 1 Antibody (D-6) is recommended for detection of Taspase 1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-Taspase 1 Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for Taspase 1 Antibody (D-6) for WB applications. This reagent is now offered in a bundle with Taspase 1 Antibody (D-6) (see ordering information below).
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Taspase 1 Antibody (D-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects Taspase 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Taspase 1 (D-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Taspase 1, also known as TASP1 or C20orf13, is a 420 amino acid endopeptidase that plays a crucial role in the cleavage of specific substrates following aspartate residues, which is essential for processing MLL (myeloid/lymphoid or mixed-lineage leukemia) and correct expression of the Hox A gene cluster. Taspase 1 is particularly important in hematopoiesis and developmental processes, and dysregulation has been implicated in various cancers. After translation, Taspase 1 undergoes autoproteolytic processing, resulting in alpha and beta subunit formation, which reassemble into a multimeric structure necessary for enzymatic activity. Taspase 1 is encoded on human chromosome 20, which comprises approximately 2% of the human genome and contains over 600 genes, some associated with serious conditions such as Creutzfeldt-Jakob disease and amyotrophic lateral sclerosis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Taspase 1 Antibody (D-6) References:
- Proteolytic cleavage of MLL generates a complex of N- and C-terminal fragments that confers protein stability and subnuclear localization. | Hsieh, JJ., et al. 2003. Mol Cell Biol. 23: 186-94. PMID: 12482972
- Taspase1: a threonine aspartase required for cleavage of MLL and proper HOX gene expression. | Hsieh, JJ., et al. 2003. Cell. 115: 293-303. PMID: 14636557
- MLL: how complex does it get? | Popovic, R. and Zeleznik-Le, NJ. 2005. J Cell Biochem. 95: 234-42. PMID: 15779005
- Crystal structure of human Taspase1, a crucial protease regulating the function of MLL. | Khan, JA., et al. 2005. Structure. 13: 1443-52. PMID: 16216576
- Uncleaved TFIIA is a substrate for taspase 1 and active in transcription. | Zhou, H., et al. 2006. Mol Cell Biol. 26: 2728-35. PMID: 16537915
- EPS15R, TASP1, and PRPF3 are novel disease candidate genes targeted by HNF4alpha splice variants in hepatocellular carcinomas. | Niehof, M. and Borlak, J. 2008. Gastroenterology. 134: 1191-202. PMID: 18395097
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Taspase 1 Antibody (D-6) | sc-514677 | 200 µg/ml | $322.00 | |||
Taspase 1 Antibody (D-6): m-IgG1 BP-HRP Bundle | sc-531788 | 200 µg Ab; 20 µg BP | $361.00 | |||
Taspase 1 Antibody (D-6) AC | sc-514677 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Taspase 1 Antibody (D-6) HRP | sc-514677 HRP | 200 µg/ml | $322.00 | |||
Taspase 1 Antibody (D-6) FITC | sc-514677 FITC | 200 µg/ml | $336.00 | |||
Taspase 1 Antibody (D-6) PE | sc-514677 PE | 200 µg/ml | $349.00 | |||
Taspase 1 Antibody (D-6) Alexa Fluor® 488 | sc-514677 AF488 | 200 µg/ml | $364.00 | |||
Taspase 1 Antibody (D-6) Alexa Fluor® 546 | sc-514677 AF546 | 200 µg/ml | $364.00 | |||
Taspase 1 Antibody (D-6) Alexa Fluor® 594 | sc-514677 AF594 | 200 µg/ml | $364.00 | |||
Taspase 1 Antibody (D-6) Alexa Fluor® 647 | sc-514677 AF647 | 200 µg/ml | $364.00 | |||
Taspase 1 Antibody (D-6) Alexa Fluor® 680 | sc-514677 AF680 | 200 µg/ml | $364.00 | |||
Taspase 1 Antibody (D-6) Alexa Fluor® 790 | sc-514677 AF790 | 200 µg/ml | $364.00 |