Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
TARDBP Double Nickase Plasmid (m): sc-433014-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TARDBP Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TARDBP Double Nickase Plasmid (m) and TARDBP Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Tardbp. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TARDBP Antibody (E-10): sc-376311
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TARDBP Double Nickase Plasmid (m) | sc-433014-NIC | 20 µg | $410.00 |
Tardbp encodes TARDBP (TDP-43), a predominantly nuclear RNA/DNA-binding protein that regulates pre-mRNA splicing, mRNA stability, transport, and microRNA biogenesis through recognition of UG-rich motifs. TARDBP participates in RNA granule dynamics and stress responses, influencing transcriptome integrity and proteostasis under cellular stress. In mouse systems, Tardbp is widely used to study neuronal and glial RNA metabolism, autoregulatory feedback on its own transcript, and cross-talk with pathways controlling nucleocytoplasmic transport and translation. Dysregulated TARDBP function and mislocalization are strongly linked to neurodegeneration and protein aggregation biology, making it a key target for mechanistic studies of RNA-binding protein pathology.
TARDBP Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Tardbp locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Tardbp. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Tardbp function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Tardbp-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.