TAPP1 Antibody (D-5): sc-374622

TAPP1 Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects TAPP1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). TAPP1 (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Tandem PH (pleckstrin homology) domain-containing protein 1 (TAPP1) serves as a crucial cytoplasmic adaptor protein that plays a significant role in cellular signaling pathways. TAPP1 is predominantly expressed in tissues such as skeletal muscle, spleen, lung, thymus, and placenta, where TAPP1 is involved in the regulation of actin-mediated membrane dynamics. Upon stimulation by growth factors and subsequent activation of phosphoinositol 3-kinase, TAPP1 is recruited to the plasma membrane, where TAPP1 accumulates in F-actin-rich membrane ruffles. This recruitment is facilitated by the specific interaction of TAPP1 C-terminal PH domain with phosphatidylinositol 3,4-bisphosphate, highlighting the importance of lipid-protein interactions in cellular signaling. Additionally, TAPP1 localization is regulated by syntrophins, which are essential for maintaining proper actin organization and membrane ruffling. Notably, overexpression of TAPP1 without concurrent syntrophin overexpression can inhibit the formation of circular ruffles, indicating a delicate balance in the regulation of cytoskeletal dynamics. Furthermore, TAPP1 may also play a role in the activation of B and T cells, underscoring TAPP1′s importance in immune responses.

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TAPP1 Antibody (D-5) References:

1. Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities.  |  Dowler, S., et al. 2000. Biochem J. 351: 19-31. PMID: 11001876
2. Crystal structure of the phosphatidylinositol 3,4-bisphosphate-binding pleckstrin homology (PH) domain of tandem PH-domain-containing protein 1 (TAPP1): molecular basis of lipid specificity.  |  Thomas, CC., et al. 2001. Biochem J. 358: 287-94. PMID: 11513726
3. Evidence that the tandem-pleckstrin-homology-domain-containing protein TAPP1 interacts with Ptd(3,4)P2 and the multi-PDZ-domain-containing protein MUPP1 in vivo.  |  Kimber, WA., et al. 2002. Biochem J. 361: 525-36. PMID: 11802782
4. TAPP1 and TAPP2 are targets of phosphatidylinositol 3-kinase signaling in B cells: sustained plasma membrane recruitment triggered by the B-cell antigen receptor.  |  Marshall, AJ., et al. 2002. Mol Cell Biol. 22: 5479-91. PMID: 12101241
5. Interaction of the protein tyrosine phosphatase PTPL1 with the PtdIns(3,4)P2-binding adaptor protein TAPP1.  |  Kimber, WA., et al. 2003. Biochem J. 376: 525-35. PMID: 14516276
6. Detection of novel intracellular agonist responsive pools of phosphatidylinositol 3,4-bisphosphate using the TAPP1 pleckstrin homology domain in immunoelectron microscopy.  |  Watt, SA., et al. 2004. Biochem J. 377: 653-63. PMID: 14604433
7. The phosphoinositol 3,4-bisphosphate-binding protein TAPP1 interacts with syntrophins and regulates actin cytoskeletal organization.  |  Hogan, A., et al. 2004. J Biol Chem. 279: 53717-24. PMID: 15485858
8. Role of the adaptor proteins Bam32, TAPP1 and TAPP2 in lymphocyte activation.  |  Allam, A. and Marshall, AJ. 2005. Immunol Lett. 97: 7-17. PMID: 15626471
9. The type Ialpha inositol polyphosphate 4-phosphatase generates and terminates phosphoinositide 3-kinase signals on endosomes and the plasma membrane.  |  Ivetac, I., et al. 2005. Mol Biol Cell. 16: 2218-33. PMID: 15716355

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-TAPP1 Antibody (D-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-TAPP1 Antibody (D-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

TAPP1 Antibody (D-5) Specifications

Alternate Product Name: TAPP1

Product Clone Name: D-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 116-195

Epitope Target: TAPP1

Epitope Species: human

Product Reactivity/Detection: TAPP1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for TAPP1 Antibody (D-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin