TAL2 Double Nickase Plasmid (m): sc-423262-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TAL2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TAL2 Double Nickase Plasmid (m) and TAL2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Tal2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAL2 Double Nickase Plasmid (m) | sc-423262-NIC | 20 µg | $410.00 | |||
TAL2 Double Nickase Plasmid (m2) | sc-423262-NIC-2 | 20 µg | $410.00 |
Mouse Tal2 encodes TAL2, a basic helix–loop–helix transcription factor that functions in gene regulatory programs by partnering with other bHLH proteins and binding E-box–containing elements to influence lineage specification and cellular differentiation. TAL2 activity is linked to transcriptional control networks that shape hematopoietic and neural developmental processes, where precise regulation of target gene expression is required for normal maturation. Dysregulated TAL family transcriptional circuitry has been implicated in aberrant proliferation and altered differentiation states in hematologic malignancy models, making Tal2 a useful locus for probing oncogenic transcription factor dependencies. Studying Tal2 in mouse systems supports mechanistic dissection of bHLH-driven transcriptional pathways, chromatin context effects, and downstream gene expression programs.
TAL2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Tal2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Tal2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Tal2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Tal2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.