Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Tak1 Double Nickase Plasmid (m): sc-424044-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Tak1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Tak1 Double Nickase Plasmid (m) and Tak1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Map3k7. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Tak1 Antibody (C-9): sc-7967
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tak1 Double Nickase Plasmid (m) | sc-424044-NIC | 20 µg | $410.00 |
Mouse Map3k7 encodes TAK1 (TGF-β-activated kinase 1), a MAP3K that integrates signals from pro-inflammatory cytokines and pattern-recognition receptors to control stress-responsive transcriptional programs. TAK1 is a central node linking upstream adaptors such as TAB proteins and TRAF complexes to activation of NF-κB, JNK, and p38 MAPK pathways, thereby regulating innate immune signaling, cell survival, apoptosis, and differentiation. In experimental models, Map3k7 perturbation reshapes inflammatory responses, epithelial and stromal homeostasis, and tissue remodeling, making it relevant to studies of immune dysregulation, fibrosis, and oncogenic signaling contexts. Its pathway position also makes it useful for dissecting crosstalk between TGF-β, TNF, IL-1, and TLR signaling modules.
Tak1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Map3k7 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Map3k7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Map3k7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Map3k7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.