TAF5L Double Nickase Plasmid (h): sc-408861-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TAF5L Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TAF5L Double Nickase Plasmid (h) and TAF5L Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TAF5L. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAF5L Double Nickase Plasmid (h) | sc-408861-NIC | 20 µg | $410.00 |
TAF5L encodes a TBP-associated factor–like protein that participates in transcription initiation by contributing to the assembly and stability of RNA polymerase II preinitiation complexes. As a component associated with TFIID/SAGA-related transcriptional regulation, TAF5L helps coordinate promoter recognition, chromatin-dependent control of gene expression, and transcriptional programs linked to cell identity and proliferation. Perturbation of TAF5L function can disrupt global transcriptional homeostasis and has been investigated in the context of transcriptional dysregulation observed in cancer and other diseases where promoter architecture and coactivator balance are altered. These features make TAF5L a useful target for mechanistic studies of core transcription factor networks and chromatin-coupled gene regulation.
TAF5L Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TAF5L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TAF5L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TAF5L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TAF5L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.