TAF1L Antibody (N3T): sc-130485
- TAF1L Antibody (N3T) is a mouse monoclonal IgG2a κ TAF1L antibody provided at 100 µg/ml
- raised against recombinant TAF1L of human origin
- recommended for detection of TAF1L of human origin by IF, IHC(P) and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for TAF1L Antibody (N3T). This work is in progress.
QUICK LINKS
SEE ALSO...
TAF1L Antibody (N3T) is a mouse monoclonal IgG2a kappa light chain antibody that detects TAF1L protein of human origin by immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-TAF1L antibody (N3T) is available as the non-conjugated form. TAF1L, or Transcription initiation factor TFIID 210 kDa subunit, is a crucial 1,826 amino acid protein that plays a vital role in spermatogenesis by serving as a functional substitute for TAF II p250 in spermatogenic cells. TAF1L (N3T) monoclonal antibody supports research into TAF1L′s ability to initiate the assembly of the preinitiation complex, which is essential for gene transcription. In somatic cells, TAF II p250, located on the X chromosome, interacts directly with the TATA promoter element to facilitate transcription. However, during male meiosis, the transcriptional activity of TAF II p250 is inactivated due to the transcriptional silencing of sex chromosomes. TAF1L, with its autosomal location, compensates for this loss of function in the testis, ensuring that transcriptional regulation continues during spermatogenesis. Notably, the gene encoding human TAF1L is believed to have originated through the retroposition of TAF II p250 mRNA during primate evolution, highlighting its evolutionary significance and functional adaptation in male reproductive biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
TAF1L Antibody (N3T) References:
- TAF(II)250: a transcription toolbox. | Wassarman, DA. and Sauer, F. 2001. J Cell Sci. 114: 2895-902. PMID: 11686293
- Functional substitution for TAF(II)250 by a retroposed homolog that is expressed in human spermatogenesis. | Wang, PJ. and Page, DC. 2002. Hum Mol Genet. 11: 2341-6. PMID: 12217962
- Identification of human TFIID components and direct interaction between a 250-kDa polypeptide and the TATA box-binding protein (TFIID tau). | Takada, R., et al. 1992. Proc Natl Acad Sci U S A. 89: 11809-13. PMID: 1465404
- Patterns of somatic mutation in human cancer genomes. | Greenman, C., et al. 2007. Nature. 446: 153-8. PMID: 17344846
- The CCG1/TAFII250 gene is mutated in thermosensitive G1 mutants of the BHK21 cell line derived from golden hamster. | Hayashida, T., et al. 1994. Gene. 141: 267-70. PMID: 8163200
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TAF1L Antibody (N3T) | sc-130485 | 100 µg/ml | $339.00 |