T84 Whole Cell Lysate: sc-364797
- 500 µg protein in 200 µl SDS-PAGE Western blotting buffer
- human whole cell lysate; colorectal carcinoma cells
- whole cell lysate provided as Western blotting positive control
- should be stored at -20°C and repeated freezing and thawing should be minimized
- sample vial should be placed at 95° C for up to 5 minutes, once prior to use
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T84 Whole Cell Lysate is derived from T84 cells, a human colorectal carcinoma cell line originally established from a lung metastasis of a colorectal cancer patient. This lysate is extensively used in gastrointestinal and cancer research to investigate the molecular mechanisms underlying colorectal cancer and epithelial cell biology. T84 cells are characterized by their ability to form tight junctions and polarized epithelial monolayers, making them an excellent model for studying epithelial barrier function and ion transport. Researchers utilize T84 Whole Cell Lysate to analyze the expression, phosphorylation, and interactions of key proteins involved in signaling pathways such as Wnt/β-catenin, PI3K/AKT, and MAPK/ERK using techniques like western blotting, immunoprecipitation, and mass spectrometry. This lysate is valuable for examining the cellular mechanisms of tumor growth, differentiation, and metastasis, particularly in the context of colorectal cancer. Additionally, T84 Whole Cell Lysate aids in studying the effects of various chemical compounds on epithelial cell signaling and barrier function, providing insights into the regulation of tight junctions and epithelial integrity. By comparing T84 lysates with those from normal colonic epithelial cells, scientists can identify specific molecular alterations associated with colorectal carcinoma, advancing research in cancer biology and epithelial cell physiology.
T84 Whole Cell Lysate References:
- Differential alteration in intestinal epithelial cell expression of toll-like receptor 3 (TLR3) and TLR4 in inflammatory bowel disease. | Cario, E. and Podolsky, DK. 2000. Infect Immun. 68: 7010-7. PMID: 11083826
- Cystic fibrosis transmembrane conductance regulator in human and mouse red blood cell membranes and its interaction with ecto-apyrase. | Sterling, KM., et al. 2004. J Cell Biochem. 91: 1174-82. PMID: 15048872
- Cystic fibrosis neutrophils have normal intrinsic reactive oxygen species generation. | McKeon, DJ., et al. 2010. Eur Respir J. 35: 1264-72. PMID: 19840964
- Physical properties and subunits of Haemopis grandis erythrocruorin. | Andonian, MR., et al. 1975. Biochim Biophys Acta. 412: 202-13. PMID: 84
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
T84 Whole Cell Lysate | sc-364797 | 500 µg/200 µl | $118.00 |