Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
T3JAM CRISPR/Cas9 KO Plasmid (h): sc-405338
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- T3JAM CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the T3JAM genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: T3JAM Antibody (B-7): sc-398781
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
T3JAM CRISPR/Cas9 KO Plasmid (h) | sc-405338 | 20 µg | $397.00 |
Overview
TRAF3IP3 (T3JAM) is an adaptor-like protein implicated in immune cell signaling, linking receptor-proximal events to downstream kinase cascades that shape activation, survival, and differentiation programs. It has been associated with regulation of MAPK and NF-κB–related processes and can influence transcriptional outputs during lymphocyte development and antigen receptor signaling. Altered TRAF3IP3 expression or function has been studied in the context of dysregulated inflammatory signaling and hematologic malignancy-associated pathways, where shifts in pathway balance can affect cellular proliferation and apoptosis. These properties make TRAF3IP3 a useful target for dissecting signal integration in hematopoietic and immune-derived model systems.
T3JAM CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TRAF3IP3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TRAF3IP3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TRAF3IP3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish T3JAM protein expression.
This CRISPR knockout system enables efficient generation of TRAF3IP3-deficient cell models for investigation of T3JAM signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TRAF3IP3 exon(s) critical for T3JAM function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TRAF3IP3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by T3JAM CRISPR/Cas9 KO Plasmid (h) and T3JAM CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TRAF3IP3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by T3JAM HDR Plasmid (h) and T3JAM HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TRAF3IP3 homology arms to support homology-directed repair at defined TRAF3IP3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.