Syntaxin 1 Antibody (HPC-1): sc-12736

Syntaxin 1 Antibody (HPC-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Syntaxin 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-Syntaxin 1 antibody (HPC-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Syntaxin 1 plays a crucial role in vesicular transport, which is vital for eukaryotic cell survival. Syntaxin 1 is primarily located on synaptic vesicle membranes and is essential for vesicle fusion with the plasma membrane, a process necessary for neurotransmitter release. Syntaxin 1 is critical for synaptic transmission, interacting with other proteins in the SNARE complex, including synaptobrevin and SNAP-25, to facilitate vesicle docking and fusion. Disruptions in Syntaxin 1 function can lead to various neurological disorders, highlighting Syntaxin 1′s importance in maintaining synaptic integrity and communication within the nervous system.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Syntaxin 1 Antibody (HPC-1) References:

1. Syntaxin 1 is required for DCC/Netrin-1-dependent chemoattraction of migrating neurons from the lower rhombic lip.  |  Cotrufo, T., et al. 2012. Eur J Neurosci. 36: 3152-64. PMID: 22946563
2. Calcium-dependent activator protein for secretion 1 (CAPS1) binds to syntaxin-1 in a distinct mode from Munc13-1.  |  Parsaud, L., et al. 2013. J Biol Chem. 288: 23050-63. PMID: 23801330
3. Calcium Promotes the Formation of Syntaxin 1 Mesoscale Domains through Phosphatidylinositol 4,5-Bisphosphate.  |  Milovanovic, D., et al. 2016. J Biol Chem. 291: 7868-76. PMID: 26884341
4. Reexamination of N-terminal domains of syntaxin-1 in vesicle fusion from central murine synapses.  |  Vardar, G., et al. 2021. Elife. 10: PMID: 34427183
5. The syntaxin family of vesicular transport receptors.  |  Bennett, MK., et al. 1993. Cell. 74: 863-73. PMID: 7690687
6. Synaptobrevin binding to synaptophysin: a potential mechanism for controlling the exocytotic fusion machine.  |  Edelmann, L., et al. 1995. EMBO J. 14: 224-31. PMID: 7835333
7. Synaptic core complex of synaptobrevin, syntaxin, and SNAP25 forms high affinity alpha-SNAP binding site.  |  McMahon, HT. and Südhof, TC. 1995. J Biol Chem. 270: 2213-7. PMID: 7836452
8. Exocytosis relating proteins in the nervous system.  |  Yamaguchi, K. and Akagawa, K. 1994. Neurosci Res. 20: 289-92. PMID: 7870381
9. Synaptic vesicle membrane fusion complex: action of clostridial neurotoxins on assembly.  |  Hayashi, T., et al. 1994. EMBO J. 13: 5051-61. PMID: 7957071
10. A role for synaptotagmin (p65) in regulated exocytosis.  |  Elferink, LA., et al. 1993. Cell. 72: 153-9. PMID: 8422678
11. Stimulation of NSF ATPase activity by alpha-SNAP is required for SNARE complex disassembly and exocytosis.  |  Barnard, RJ., et al. 1997. J Cell Biol. 139: 875-83. PMID: 9362506
12. Structural organization of the synaptic exocytosis core complex.  |  Lin, RC. and Scheller, RH. 1997. Neuron. 19: 1087-94. PMID: 9390521

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Syntaxin 1 Antibody (HPC-1) has 85 citations in a variety of scientific publications.

Primary antibodies like Anti-Syntaxin 1 Antibody (HPC-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Syntaxin 1 Antibody (HPC-1) Specifications

Alternate Product Name: Syntaxin 1

Product Clone Name: HPC-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Syntaxin 1

Epitope Species: rat

Product Reactivity/Detection: Syntaxin 1A and Syntaxin 1B

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 85 citations in various scientific publications

Available Conjugates/Forms for Syntaxin 1 Antibody (HPC-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin