Synapsin IIa Double Nickase Plasmid (m): sc-423234-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Synapsin IIa Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Synapsin IIa Double Nickase Plasmid (m) and Synapsin IIa Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Syn2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Synapsin IIa Antibody (1): sc-136086
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Synapsin IIa Double Nickase Plasmid (m) | sc-423234-NIC | 20 µg | $410.00 | |||
Synapsin IIa Double Nickase Plasmid (m2) | sc-423234-NIC-2 | 20 µg | $410.00 |
Mouse Syn2 encodes synapsin IIa, a synaptic vesicle–associated phosphoprotein that regulates neurotransmitter release by tethering vesicles to the actin cytoskeleton and controlling vesicle mobilization from the reserve pool. Synapsin IIa function is modulated by activity-dependent phosphorylation downstream of kinases such as PKA, CaMKs, and MAPK/ERK, linking neuronal firing to presynaptic plasticity and vesicle cycling. Through its role in synaptogenesis and short-term synaptic dynamics, Syn2 contributes to the maintenance of excitatory and inhibitory circuit balance. Dysregulation of synapsin pathways has been associated with neurodevelopmental and neuropsychiatric phenotypes, making Syn2 a relevant target for studying mechanisms underlying altered synaptic transmission.
Synapsin IIa Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Syn2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Syn2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Syn2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Syn2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.