Sva CRISPR/Cas9 KO Plasmid (m): sc-423225
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sva CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sva genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sva CRISPR/Cas9 KO Plasmid (m) | sc-423225 | 20 µg | $397.00 |
Overview
Sva (steroidogenic factor 1–associated) is a nuclear protein implicated in the regulation of steroidogenic gene expression through interactions with transcriptional programs governing endocrine development and hormone biosynthesis. In mouse cells, Sva is linked to modulation of nuclear receptor–dependent transcription, influencing pathways that integrate chromatin context with tissue-specific gene regulation. Altered control of steroidogenic transcriptional networks can affect gonadal and adrenal differentiation and downstream endocrine homeostasis, making Sva a relevant target for mechanistic studies of developmental and metabolic phenotypes. Loss- or gain-of-function interrogation of Sva supports dissection of transcriptional circuitry that couples cellular differentiation states to hormone-responsive signaling.
Sva CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sva gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sva together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sva open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sva protein expression.
This CRISPR knockout system enables efficient generation of Sva-deficient cell models for investigation of Sva signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Sva exon(s) critical for Sva function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Sva genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sva CRISPR/Cas9 KO Plasmid (m) and Sva CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sva locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sva HDR Plasmid (m) and Sva HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sva homology arms to support homology-directed repair at defined Sva target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.