Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
SUZ12 Double Nickase Plasmid (m): sc-424636-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SUZ12 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SUZ12 Double Nickase Plasmid (m) and SUZ12 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Suz12. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SUZ12 Antibody (D-10): sc-271325
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SUZ12 Double Nickase Plasmid (m) | sc-424636-NIC | 20 µg | $410.00 |
Suz12 encodes SUZ12, an essential core component of Polycomb repressive complex 2 (PRC2) that cooperates with EZH2/EED to deposit the repressive histone mark H3K27me3 and establish heritable gene silencing. In mouse cells, SUZ12 helps regulate developmental gene programs, lineage commitment, and chromatin architecture by controlling transcriptional repression at bivalent promoters and other Polycomb target loci. Through PRC2-dependent epigenetic regulation, SUZ12 influences processes such as stem cell self-renewal, differentiation, and cell cycle control, and its perturbation is linked to dysregulated transcriptional states relevant to cancer biology and developmental phenotypes. These functions make Suz12 a widely used node for studying chromatin-mediated repression, enhancer–promoter regulation, and context-specific gene expression networks.
SUZ12 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Suz12 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Suz12. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Suz12 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Suz12-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.