Sulpho NHS biotin CAS: 119616-38-5
MF: C14H18N3O8S2Na
MW: 443.42
A water-soluble biotinylation reagent.

Sulpho NHS biotin (CAS 119616-38-5)

Sulpho NHS biotin | CAS 119616-38-5 is rated 5.0 out of 5 by 1.
  • y_2020, m_5, d_30, h_14
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.8
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_258187, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 195ms
  • REVIEWS, PRODUCT
5
1
4
0
3
0
2
0
1
0
Alternate Names: Sulfosuccinimidyl biotin
Application: Sulpho NHS biotin is a water-soluble biotinylation reagent
CAS Number: 119616-38-5
Molecular Weight: 443.42
Molecular Formula: C14H18N3O8S2Na
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

Sulpho NHS biotin (Sulfosuccinimidyl biotin) is a water soluble biotinylation reagent used for simple and efficient labeling of antibodies, proteins and primary amine-containing molecules. Biotin is a vitamin which binds with high affinity to avidin and streptavidin proteins. Biotin is small (244 Da), and may be conjugated to proteins without altering their biological activities. NHS-activated biotins couple to primary amino groups (-NH2) in pH 7-9 buffers to form stable amide bonds. Sulpho NHS biotin has also been used for labeling of membrane proteins; due to the charge of the sodium sulfoxide group on the succinimidyl ring, it cannot permeate the cell membrane. If the cell remains intact, only primary amines exposed on the surface will be biotinylated. Recommended for use with avidin-conjugated fluorescent molecules: avidin-FITC, sc-2865; avidin-PE, sc-2876; avidin-APC, sc-2898; avidin-PE-Cy7, sc-3769; avidin-APC-Cy7, sc-3780; DY-550-avidin, sc-221567; DY-630-avidin, sc-221572.


References

1. Yamamoto, A.M., et al. 1993. Clin. Exp. Immunol. 92: 381-390. PMID: 7685669
2. Pintado, C.O., et al. 1995. Vet. Immunol. Immunopathol. 44: 359-368. PMID: 7538249
3. Coonrod, S., et al. 1999. Mol. Hum. Reprod. 5: 1027-1033. PMID: 10541564
4. Hewett, P.W. 2001. Int. J. Biochem. Cell Biol. 33: 325-335. PMID: 11312103

Physical State :
Solid
Solubility :
Soluble in water (10 mg/ml), and DMF (50 mg/ml).
Storage :
Store at 4° C
Refractive Index :
n20D ~1.65 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
23667738
MDL Number :
MFCD00078535
SMILES :
C1[[email protected]]2[[email protected]@H](C(S1)CCCCC(=O)ON3C(=O)CC(C3=O)S(=O)(=O)[O-])NC(=O)N2.[Na+]

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 323ms
  • QUESTIONS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
Rated 5 out of 5 by from Numerous publications speak about the use Numerous publications speak about the use of Sulpho NHS biotin, a biotinylation reagent, to label antibodies and proteins. -SCBT Publication Review
Date published: 2015-03-16
  • y_2020, m_5, d_30, h_14
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.8
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_258187, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 89ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2020 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.