Sulfo-SMCC (CAS 92921-24-9)

Sulfo-SMCC, a heterobifunctional protein crosslinker, possesses sulfhydryl- and amine-reactive properties along with an 11.6 Angstrom spacer arm. This versatile chemical compound finds wide application in biochemical and physiological research. As a water-soluble, thiol-reactive compound, Sulfo-SMCC has demonstrated its efficacy across multiple applications. It enables the covalent attachment of proteins to solid supports during protein purification. It facilitates the identification and quantification of protein-protein interactions. Furthermore, it contributes to the study of protein-DNA interactions by examining the binding between DNA and proteins. In cell-based assays, Sulfo-SMCC aids in the exploration of cell adhesion and the binding of cell surface receptors. Additionally, it plays a role in the synthesis of liposomes, nanoparticles, and other nanomaterials. The covalent binding of Sulfo-SMCC to thiol groups on proteins and other molecules occurs via a nucleophilic substitution mechanism. Specifically, the electrophilic carbon of the Sulfo-SMCC molecule reacts with the sulfhydryl group of the protein, forming a stable covalent bond. In summary, Sulfo-SMCC serves as a sulfhydryl- and amine-reactive, heterobifunctional protein crosslinker, featuring an 11.6 Angstrom spacer arm. Its water solubility and thiol reactivity make it invaluable in protein-related research.