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Suc-Ala-Ala-Ala-pNA (CAS 52299-14-6)

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Alternate Names:
N-Succinyl-Ala-Ala-Ala-p-nitroanilide; N-Succinyl-L-alanyl-L-alanyl-L-alanine 4-nitroanilide
CAS Number:
52299-14-6
Molecular Weight:
451.43
Molecular Formula:
C19H25N5O8
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Suc-Ala-Ala-Ala-pNA is a synthetic peptide used as a chromogenic substrate in biochemistry research for the assay and characterization of proteolytic enzymes, particularly those with trypsin-like activity. When these enzymes cleave the peptide bond after the alanine residue, the p-nitroanilide (pNA) group is released, resulting in a yellow color that can be quantitatively measured using spectrophotometry. This allows for the monitoring of enzyme kinetics and the determination of enzyme activity under various conditions. Additionally, Suc-Ala-Ala-Ala-pNA is employed in the identification and study of protease inhibitors by observing the decrease in colorimetric response upon inhibitor binding. The straightforward and sensitive nature of this assay makes Suc-Ala-Ala-Ala-pNA useful in enzymology for investigating the specificity and regulation of proteolytic enzymes, as well as screening for potential enzyme inhibitors.


Suc-Ala-Ala-Ala-pNA (CAS 52299-14-6) References

  1. Enzymatic characterization of a cubilin-related serine proteinase from the hard tick Haemaphysalis longicornis.  |  Miyoshi, T., et al. 2004. J Vet Med Sci. 66: 1195-8. PMID: 15528848
  2. Kinetics of nitroanilide cleavage by astacin.  |  Stöcker, W., et al. 1991. Biol Chem Hoppe Seyler. 372: 385-92. PMID: 1910577
  3. Characterization of elastase associated with granulomatous tissue remodeling.  |  Izaki, S., et al. 1986. J Cell Biochem. 32: 79-89. PMID: 2429974
  4. Interference of cartilage surface with interaction of granulocyte elastase with alpha 1-proteinase inhibitor. An in vitro model of enzyme inhibition in the joint space.  |  Burkhardt, H., et al. 1987. Rheumatol Int. 7: 133-8. PMID: 3313660
  5. Case report: Five-year periodontal management of a patient with two novel mutation sites in ELANE-induced cyclic neutropenia.  |  Lao, Z., et al. 2022. Front Genet. 13: 972598. PMID: 36386806

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Suc-Ala-Ala-Ala-pNA, 5 mg

sc-473488
5 mg
$67.00

Suc-Ala-Ala-Ala-pNA, 100 mg

sc-473488A
100 mg
$240.00