STK16 CRISPR/Cas9 KO Plasmid (m): sc-423194
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- STK16 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the STK16 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
STK16 CRISPR/Cas9 KO Plasmid (m) | sc-423194 | 20 µg | $397.00 |
Overview
Mouse Stk16 encodes serine/threonine kinase 16 (STK16), a constitutively active kinase enriched at Golgi and membrane-associated compartments that contributes to protein trafficking, Golgi organization, and regulation of secretory pathway dynamics. STK16 activity has been linked to control of cell growth and survival programs, with reported crosstalk to signaling nodes that influence proliferation and stress responses. By modulating phosphorylation-dependent processes, STK16 can shape intracellular transport and cell-cycle–associated phenotypes relevant to studies of tissue homeostasis. Dysregulated kinase signaling and altered trafficking networks are frequently observed in disease-associated cellular states, making Stk16 a useful target for mechanistic interrogation of pathway dependencies in mouse models.
STK16 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Stk16 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Stk16 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Stk16 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish STK16 protein expression.
This CRISPR knockout system enables efficient generation of Stk16-deficient cell models for investigation of STK16 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Stk16 exon(s) critical for STK16 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Stk16 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by STK16 CRISPR/Cas9 KO Plasmid (m) and STK16 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Stk16 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by STK16 HDR Plasmid (m) and STK16 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Stk16 homology arms to support homology-directed repair at defined Stk16 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.