STEAP2 Double Nickase Plasmid (m): sc-428702-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- STEAP2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- STEAP2 Double Nickase Plasmid (m) and STEAP2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Steap2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
STEAP2 Double Nickase Plasmid (m) | sc-428702-NIC | 20 µg | $410.00 | |||
STEAP2 Double Nickase Plasmid (m2) | sc-428702-NIC-2 | 20 µg | $410.00 |
Steap2 encodes six-transmembrane epithelial antigen of the prostate 2 (STEAP2), a membrane-associated metalloreductase implicated in iron and copper homeostasis through reduction of ferric and cupric ions to facilitate cellular metal uptake. In mouse cells, STEAP2 localizes to endosomal and plasma membrane compartments and contributes to redox regulation, vesicular trafficking, and metabolic processes linked to metal-dependent enzymes. Altered STEAP family activity has been connected to oxidative stress, dysregulated proliferation, and changes in differentiation programs in multiple tissue contexts. Steap2 is therefore of interest for studying metal ion handling pathways and their impact on cellular signaling and disease-associated phenotypes in vivo and in vitro.
STEAP2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Steap2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Steap2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Steap2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Steap2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.