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Staurosporine (CAS 62996-74-1)

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Alternate Names:
Staurosporine is also known as Staurosporin and Antibiotic AM-2282.
Application:
Staurosporine is an antifungal inhibitor of many different kinases via interaction with the ATP binding site
CAS Number:
62996-74-1
Purity:
≥95%
Molecular Weight:
466.53
Molecular Formula:
C28H26N4O3
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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Staurosporine, also referred to as Antibiotic AM-2282, is a microbial alkaloid with antifungal activity. Staurosporine may act to inhibit a variety of kinases via interaction with the ATP binding site. Staurosporine induces association of purified PKC with inside-out vesicles from erythrocyte membranes and augments PMA-induced ornithine decarboxylase. Staurosporine also activates a bcl-2-regulated apoptosis pathway. Kinases inhibited by Staurosporine include PKA (Ki=7.0 nM), PKG (Ki=8.5 nM), MYLK (MLCK, Ki=1.3 nM), PKC (Ki=0.7 nM), CaMK (IC50=20 nM), CAMKII (Ki=20nM), tyrosine kinases (IC50=70 nM) and phosphorylase kinase (IC50=0.5 nM). Further, there is evidence that Staurosporine inhibits c-Src, Cdc2, Cdk2, Cdk4, Cdk5, cGKI, cyclin A, cyclin B, cyclin D, Fgr, GSK-3 beta, Lyn, p35, Pim-3, PKC alpha, Syk and Topo II.


Staurosporine (CAS 62996-74-1) References

  1. Changes in mitochondrial membrane potential during staurosporine-induced apoptosis in Jurkat cells.  |  Scarlett, JL., et al. 2000. FEBS Lett. 475: 267-72. PMID: 10869569
  2. Molecular mechanism of staurosporine-induced apoptosis in osteoblasts.  |  Chae, HJ., et al. 2000. Pharmacol Res. 42: 373-81. PMID: 10987998
  3. Staurosporine induces apoptosis through both caspase-dependent and caspase-independent mechanisms.  |  Belmokhtar, CA., et al. 2001. Oncogene. 20: 3354-62. PMID: 11423986
  4. Mechanism of staurosporine-induced apoptosis in murine hepatocytes.  |  Feng, G. and Kaplowitz, N. 2002. Am J Physiol Gastrointest Liver Physiol. 282: G825-34. PMID: 11960779
  5. Staurosporine induces apoptosis of melanoma by both caspase-dependent and -independent apoptotic pathways.  |  Zhang, XD., et al. 2004. Mol Cancer Ther. 3: 187-97. PMID: 14985459
  6. Staurosporine is a potent inhibitor of p34cdc2 and p34cdc2-like kinases.  |  Gadbois, DM., et al. 1992. Biochem Biophys Res Commun. 184: 80-5. PMID: 1567459
  7. Staurosporine: an effective inhibitor for Ca2+/calmodulin-dependent protein kinase II.  |  Yanagihara, N., et al. 1991. J Neurochem. 56: 294-8. PMID: 1846174
  8. Staurosporine inhibits the soluble and membrane-bound protein tyrosine kinases of human neutrophils.  |  Badwey, JA., et al. 1991. Biochem Biophys Res Commun. 178: 423-9. PMID: 1859401
  9. Staurosporine inhibits a tyrosine protein kinase in human hepatoma cell membranes.  |  Fallon, RJ. 1990. Biochem Biophys Res Commun. 170: 1191-6. PMID: 2167672
  10. K252a is a potent and selective inhibitor of phosphorylase kinase.  |  Elliott, LH., et al. 1990. Biochem Biophys Res Commun. 171: 148-54. PMID: 2393389
  11. Staurosporine, a protein kinase C inhibitor interferes with proliferation of arterial smooth muscle cells.  |  Matsumoto, H. and Sasaki, Y. 1989. Biochem Biophys Res Commun. 158: 105-9. PMID: 2912441
  12. The protein kinase inhibitor staurosporine, like phorbol esters, induces the association of protein kinase C with membranes.  |  Wolf, M. and Baggiolini, M. 1988. Biochem Biophys Res Commun. 154: 1273-9. PMID: 3408497
  13. Staurosporine, a potent inhibitor of phospholipid/Ca++dependent protein kinase.  |  Tamaoki, T., et al. 1986. Biochem Biophys Res Commun. 135: 397-402. PMID: 3457562
  14. Protein kinase inhibition by staurosporine revealed in details of the molecular interaction with CDK2.  |  Lawrie, AM., et al. 1997. Nat Struct Biol. 4: 796-801. PMID: 9334743
  15. Calcium and reactive oxygen species mediate staurosporine-induced mitochondrial dysfunction and apoptosis in PC12 cells.  |  Kruman, I., et al. 1998. J Neurosci Res. 51: 293-308. PMID: 9486765
  16. Staurosporine-induced apoptosis in cardiomyocytes: A potential role of caspase-3.  |  Yue, TL., et al. 1998. J Mol Cell Cardiol. 30: 495-507. PMID: 9515027

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Staurosporine, 100 µg

sc-3510
100 µg
$82.00

Staurosporine, 1 mg

sc-3510A
1 mg
$150.00

Staurosporine, 5 mg

sc-3510B
5 mg
$388.00

How should this be resuspended? I tried looking at the the SDS, yet there seemed to be not too much on resuspension.

Asked by: 3jpcwick
Thank you for your question . In DMSO at -20C
Answered by: BlakeJ
Date published: 2023-02-23

I had use the staurosporine to induce apoptosis on my target cells but the number of cells undergoes apoptosis is not alot even in high concentration. I would like to ask whether the staurosporine needs to be warm up prior to usage in order for it to work

Asked by: Alyssa
For protocol inquiry Please contact Technical Service.
Answered by: Tech Sales
Date published: 2021-06-25

Once dissolved in DMSO, how long is this stable for?

Asked by: 13punMA
Thank you for asking about our Staurosporine (CAS# 62996-74-1). The chemical is stable for a few months when stored at -20ºC. Stock solutions can be stable for up to 6 months when stored at -70ºC.
Answered by: Chemical Support 8
Date published: 2017-03-01

What is the chemical grade for this product, sc-3510?

Asked by: SCM4
Thank you for your question. We do not offer a specific grade for Staurosporine (CAS 62996-74-1): sc-3510, however the purity is ≥99%.
Answered by: Technical Support
Date published: 2016-12-14

After resuspending using DMSO, how should the solution be stored?

Asked by: chemicalsmg
Thank you for your question. If necessary to store Staurosporine (CAS 62996-74-1) as resuspension, aliquot out and store at -20ºC. Avoid repeated freeze/thaw cycles.
Answered by: Technical Support
Date published: 2016-12-14
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Rated 5 out of 5 by from Staurosporine, 100 ugStaurosporine was purchased from Santa Cruz Biotechnology on May 8, 2017. The compound was reconstituted in DMSO. Human AML cells line MOLM13 was treated in the growth medium containing 100 nM and 500 nM of staurosporine respectively at 37C for 24h. Western blotting was performed for characterization of caspase 3. Conclusion: Staurosporine effectively stimulates the activation process of caspase 3 in MOLM13 cells.
Date published: 2017-06-13
Rated 5 out of 5 by from Satisfied with productSatisfied with product.
Date published: 2015-10-07
Rated 5 out of 5 by from No commentNo comment.
Date published: 2015-04-13
Rated 5 out of 5 by from Broad enzymatic inhibitor and also inhibitorBroad enzymatic inhibitor and also inhibitor of a variety of kinases. In order to inhibit, staurosporine interferes with the ATP binding site. -SCBT QC
Date published: 2015-02-11
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Staurosporine is rated 5.0 out of 5 by 4.
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