Stains-all (CAS 7423-31-6)
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Stains-all is a cationic carbocyanine dye used for differential staining of nuclei acids and proteins with RNA staining blue/purple, DNA staining blue, and proteins staining red. In addition, the dye can be prepared by dissolving Stains-All in formamide and buffer. It has been reported that Stains-all is 50 to 100% more intense than Coomassie blue (sc-294100) in staining proteins such as Ca2+-binding proteins in muscle extracts. Furthermore, Stains-all is an ideal dye for the visualization and identification of proteins on polyacrylamide gels because it differentiates between highly acidic proteins which stain blue and less acidic proteins which stain pink. When employed on polyacrylamide gels, the intensity of Stains-all can be increased by the addition of silver nitrate (sc-203378).
Stains-all (CAS 7423-31-6) References
- Detection of submicrogram quantities of glycosaminoglycans on agarose gels by sequential staining with toluidine blue and Stains-All. | Volpi, N. and Maccari, F. 2002. Electrophoresis. 23: 4060-6. PMID: 12481260
- Application of stains-all staining to the analysis of axonemal tubulins: identification of beta-tubulin and beta-isotubulins. | Nakamura, K., et al. 1990. J Biochem Biophys Methods. 21: 237-45. PMID: 1704028
- Application of stains-all for demarcation of cement lines in methacrylate embedded bone. | Gruber, HE. and Mekikian, P. 1991. Biotech Histochem. 66: 181-4. PMID: 1716999
- Improved staining of phosphoproteins with high sensitivity in polyacrylamide gels using Stains-All. | Cong, WT., et al. 2013. Electrophoresis. 34: 3277-86. PMID: 24114871
- A color-code for glycosaminoglycans identification by means of polyacrylamide gel electrophoresis stained with the cationic carbocyanine dye Stains-all. | Andrade, JPS., et al. 2018. Electrophoresis. 39: 666-669. PMID: 29105785
- Distinction between glycomacropeptide and β-lactoglobulin with 'stains all' dye on tricine SDS-PAGE gels. | Sharma, N., et al. 2021. Food Chem. 340: 127923. PMID: 32889212
- Designing topical hyaluronic acid technology-Size does matter…. | Widgerow, AD., et al. 2022. J Cosmet Dermatol. 21: 2865-2870. PMID: 35486441
- Staining of the Ca2+-binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationic carbocyanine dye 'Stains-all'. | Campbell, KP., et al. 1983. J Biol Chem. 258: 11267-73. PMID: 6193121
- Studies on the interaction of the dye, stains-all, with individual calcium-binding domains of calmodulin. | Sharma, Y., et al. 1993. FEBS Lett. 326: 59-64. PMID: 8325390
- The staining of acidic proteins on polyacrylamide gels: enhanced sensitivity and stability of 'Stains-all' staining in combination with silver nitrate. | Goldberg, HA. and Warner, KJ. 1997. Anal Biochem. 251: 227-33. PMID: 9299020
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Stains-all, 1 g | sc-215902 | 1 g | $110.00 | |||
Stains-all, 5 g | sc-215902A | 5 g | $365.00 |