St3Gal-II Antibody (34-K): sc-100856

St3Gal-II Antibody (34-K) is a mouse monoclonal IgG2a kappa light chain antibody that detects St3Gal-II protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-St3Gal-II antibody (34-K) is available as non-conjugated format. St3Gal-II, also known as SIAT4B, Gal-NAc6S, ST3GAL2, or ST3GalA.2, is a member of the glycosyltransferase 29 family and plays a crucial role in biosynthesis of sialylated glycoconjugates, which are essential for various biological processes, including cell signaling and immune response. St3Gal-II is predominantly expressed in heart and skeletal muscle, where St3Gal-II is localized to the Golgi apparatus as a single-pass membrane protein. The unique structure of St3Gal-II allows St3Gal-II to catalyze both forward and reversible sialylation reactions, facilitating the transfer of sialic acid from cytidine 5′-monophosphate to galactose-containing substrates, as well as regenerating CMP-NeuAc for further glycosylation processes. The ability of St3Gal-II to modify glycoproteins and glycolipids with sialic acid is vital for maintaining cellular interactions and modulating immune response, making St3Gal-II an important target for research in developmental biology and disease mechanisms.

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St3Gal-II Antibody (34-K) References:

1. Genomic structure, expression, and transcriptional regulation of human Gal beta 1,3 GalNAc alpha 2,3-sialyltransferase gene.  |  Taniguchi, A., et al. 2003. Biochem Biophys Res Commun. 300: 570-6. PMID: 12504121
2. Human alpha2,3-sialyltransferase (ST3Gal II) is a stage-specific embryonic antigen-4 synthase.  |  Saito, S., et al. 2003. J Biol Chem. 278: 26474-9. PMID: 12716912
3. Construction of highly glycosylated mucin-type glycopeptides based on microwave-assisted solid-phase syntheses and enzymatic modifications.  |  Matsushita, T., et al. 2006. J Org Chem. 71: 3051-63. PMID: 16599599
4. The evolution of galactose alpha2,3-sialyltransferase: Ciona intestinalis ST3GAL I/II and Takifugu rubripes ST3GAL II sialylate Galbeta1,3GalNAc structures on glycoproteins but not glycolipids.  |  Lehmann, F., et al. 2008. Glycoconj J. 25: 323-34. PMID: 17973185
5. Reversible sialylation: synthesis of cytidine 5'-monophospho-N-acetylneuraminic acid from cytidine 5'-monophosphate with alpha2,3-sialyl O-glycan-, glycolipid-, and macromolecule-based donors yields diverse sialylated products.  |  Chandrasekaran, EV., et al. 2008. Biochemistry. 47: 320-30. PMID: 18067323
6. Critical role of evolutionarily conserved glycosylation at Asn211 in the intracellular trafficking and activity of sialyltransferase ST3Gal-II.  |  Ruggiero, FM., et al. 2015. Biochem J. 469: 83-95. PMID: 25916169
7. Mice lacking sialyltransferase ST3Gal-II develop late-onset obesity and insulin resistance.  |  Lopez, PH., et al. 2017. Glycobiology. 27: 129-139. PMID: 27683310
8. One-pot multienzyme (OPME) chemoenzymatic synthesis of brain ganglioside glycans with human ST3GAL II expressed in E. coli.  |  Yang, X., et al. 2022. ChemCatChem. 14: PMID: 35784007
9. Human ST3Gal II and ST6GalNAc IV genes increase human serum-mediated cytotoxicity to xenogeneic cells.  |  Choi, H., et al. 2024. Xenotransplantation. 31: exen12855. PMID: 38602029
10. Three genes that encode human beta-galactoside alpha 2,3-sialyltransferases. Structural analysis and chromosomal mapping studies.  |  Chang, ML., et al. 1995. Glycobiology. 5: 319-25. PMID: 7655169
11. Molecular cloning and expression of human Gal beta 1,3GalNAc alpha 2,3-sialytransferase (hST3Gal II).  |  Kim, YJ., et al. 1996. Biochem Biophys Res Commun. 228: 324-7. PMID: 8920913
12. Cloning and expression of cDNA for a human Gal(beta1-3)GalNAc alpha2,3-sialyltransferase from the CEM T-cell line.  |  Giordanengo, V., et al. 1997. Eur J Biochem. 247: 558-66. PMID: 9266697

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-St3Gal-II Antibody (34-K) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-St3Gal-II Antibody (34-K) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

St3Gal-II Antibody (34-K) Specifications

Alternate Product Name: St3Gal-II

Product Clone Name: 34-K

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 28-128

Epitope Target: St3Gal-II

Epitope Species: human

Product Reactivity/Detection: St3Gal-II

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for St3Gal-II Antibody (34-K): N/A