St3Gal-I Double Nickase Plasmid (m): sc-422935-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- St3Gal-I Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- St3Gal-I Double Nickase Plasmid (m) and St3Gal-I Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting St3gal1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
St3Gal-I Double Nickase Plasmid (m) | sc-422935-NIC | 20 µg | $410.00 | |||
St3Gal-I Double Nickase Plasmid (m2) | sc-422935-NIC-2 | 20 µg | $410.00 |
Mouse St3gal1 encodes the Golgi-resident sialyltransferase St3Gal-I, which catalyzes the transfer of sialic acid in an α2,3 linkage to Galβ1-3GalNAc on core 1 O-glycans, shaping the sialylation state of mucin-type glycoproteins. By modulating terminal glycan structures, St3Gal-I influences glycoprotein stability, receptor–ligand interactions, and cell–cell or cell–matrix adhesion processes central to immune regulation and epithelial biology. This enzyme is a key component of O-glycosylation pathways that affect membrane trafficking and signaling microenvironments, with downstream impacts on inflammation and tumor-associated glycan remodeling. Altered ST3GAL1 activity and α2,3-sialylated epitopes are frequently studied in contexts such as immune cell development, metastasis-associated adhesion, and glyco-immune evasion mechanisms.
St3Gal-I Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the St3gal1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within St3gal1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt St3gal1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of St3gal1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.