SSTR1 Double Nickase Plasmid (h): sc-403410-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SSTR1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SSTR1 Double Nickase Plasmid (h) and SSTR1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SSTR1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SSTR1 Antibody (1F7): sc-293490
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SSTR1 Double Nickase Plasmid (h) | sc-403410-NIC | 20 µg | $410.00 | |||
SSTR1 Double Nickase Plasmid (h2) | sc-403410-NIC-2 | 20 µg | $410.00 |
SSTR1 encodes somatostatin receptor 1, a Gi/o-coupled GPCR that binds somatostatin peptides to suppress adenylyl cyclase activity, reduce cAMP production, and modulate downstream effectors such as MAPK/ERK and PI3K signaling. Through these pathways, SSTR1 influences calcium flux, ion channel activity, and transcriptional programs that regulate neuronal excitability, endocrine secretion, and cell-cycle control. Receptor signaling contributes to coordinated feedback in neuroendocrine and gastrointestinal systems, where somatostatin tone shapes hormone release and synaptic transmission. Dysregulated somatostatin receptor expression or signaling has been associated with altered neuroendocrine differentiation and proliferative phenotypes, supporting its use as a mechanistic target in studies of tumor biology and neural/endocrine regulation.
SSTR1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SSTR1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SSTR1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SSTR1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SSTR1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.