SSH1 Double Nickase Plasmid (m): sc-433087-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SSH1 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SSH1 Double Nickase Plasmid (m) and SSH1 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ssh1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SSH1 Double Nickase Plasmid (m) | sc-433087-NIC | 20 µg | $410.00 | |||
SSH1 Double Nickase Plasmid (m2) | sc-433087-NIC-2 | 20 µg | $410.00 |
Mouse SSH1 (Slingshot-1) is a dual-specificity phosphatase that regulates actin cytoskeleton remodeling by dephosphorylating and reactivating cofilin, promoting F-actin turnover and directional cell motility. Through integration with Rho family GTPase signaling, LIMK/cofilin pathways, and stress-responsive phosphatase networks, SSH1 helps coordinate lamellipodia dynamics, neurite outgrowth, and adhesion-dependent migration. Altered SSH1 activity has been linked to dysregulated cytoskeletal organization and impaired cell movement phenotypes that are relevant to studies of neurodevelopment, inflammation, and tumor cell invasion models. In mouse systems, SSH1 is therefore frequently investigated for its role in mechanotransduction, synaptic plasticity, and migration-dependent tissue remodeling.
SSH1 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ssh1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ssh1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ssh1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ssh1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.