SRPK2 Antibody (A-5): sc-390930
- SRPK2 Antibody (A-5) is a mouse monoclonal mouse IgG2a κ κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 10-37 at the N-terminus of SRPK2 of human origin
- recommended for detection of SRPK2 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine and porcine
- m-IgG2a BP-HRP is the preferred secondary detection reagent for SRPK2 Antibody (A-5) for WB applications. This reagent is now offered in a bundle with SRPK2 Antibody (A-5) (see ordering information below).
QUICK LINKS
SRPK2 Antibody (A-5) is a mouse monoclonal IgG2a antibody that detects SRPK2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SRPK2, a member of the serine/arginine-rich protein kinase family, plays a crucial role in the regulation of pre-mRNA splicing by specifically phosphorylating serine/arginine-rich (SR) proteins, which are essential for both constitutive and alternative splicing processes. The phosphorylation of SR proteins by SRPK2 is vital for the coordination of splicing with transcription, ensuring accurate gene expression regulation. SRPK2 is predominantly expressed in the brain, where anti-SRPK2 antibody (A-5) can detect splicing events necessary for neuronal function, while also being moderately present in heart and skeletal muscle tissues. A proline-rich sequence at the amino terminus enhances specificity and function in splicing regulation. SRPK2 monoclonal antibody (A-5) serves as an invaluable tool for researchers studying the intricate mechanisms of gene expression and the role of splicing in various biological processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SRPK2 Antibody (A-5) References:
- Localization of serine kinases, SRPK1 (SFRSK1) and SRPK2 (SFRSK2), specific for the SR family of splicing factors in mouse and human chromosomes. | Wang, HY., et al. 1999. Genomics. 57: 310-5. PMID: 10198174
- Identification of SRPK1 and SRPK2 as the major cellular protein kinases phosphorylating hepatitis B virus core protein. | Daub, H., et al. 2002. J Virol. 76: 8124-37. PMID: 12134018
- Suppression of hepatitis B virus replication by SRPK1 and SRPK2 via a pathway independent of the phosphorylation of the viral core protein. | Zheng, Y., et al. 2005. Virology. 342: 150-8. PMID: 16122776
- Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4/U6-U5 tri-snRNP into the spliceosome. | Mathew, R., et al. 2008. Nat Struct Mol Biol. 15: 435-43. PMID: 18425142
- Abnormal expression of the pre-mRNA splicing regulators SRSF1, SRSF2, SRPK1 and SRPK2 in non small cell lung carcinoma. | Gout, S., et al. 2012. PLoS One. 7: e46539. PMID: 23071587
- An RS motif within the Epstein-Barr virus BLRF2 tegument protein is phosphorylated by SRPK2 and is important for viral replication. | Duarte, M., et al. 2013. PLoS One. 8: e53512. PMID: 23326445
- Paraquat modulates alternative pre-mRNA splicing by modifying the intracellular distribution of SRPK2. | Vivarelli, S., et al. 2013. PLoS One. 8: e61980. PMID: 23613995
- Delta-Secretase Phosphorylation by SRPK2 Enhances Its Enzymatic Activity, Provoking Pathogenesis in Alzheimer's Disease. | Wang, ZH., et al. 2017. Mol Cell. 67: 812-825.e5. PMID: 28826672
- Serine-Arginine Protein Kinase SRPK2 Modulates the Assembly of the Active Zone Scaffolding Protein CAST1/ERC2. | Arancibia, D., et al. 2019. Cells. 8: PMID: 31671734
- SRPK2: a differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells. | Wang, HY., et al. 1998. J Cell Biol. 140: 737-50. PMID: 9472028
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SRPK2 Antibody (A-5) | sc-390930 | 200 µg/ml | $322.00 | |||
SRPK2 Antibody (A-5): m-IgG2a BP-HRP Bundle | sc-546075 | 200 µg Ab; 10 µg BP | $361.00 | |||
SRPK2 (A-5) Neutralizing Peptide | sc-390930 P | 100 µg/0.5 ml | $69.00 |