Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
SRP68 Antibody (4-YD13) is a high quality monoclonal SRP68 antibody (also designated SRP68 antibody) suitable for the detection of the SRP68 protein of human origin. SRP68 Antibody (4-YD13) is available as the non-conjugated anti-SRP68 antibody. Signal recognition particle (SRP) is a ribonucleoprotein composed of an Alu domain and an S domain that contains six proteins. The S domain contains a unique sequence of SRP RNA and four SRP proteins: SRP19, SRP54, SRP68 and SRP72. The Alu domain contains two SRP proteins: SRP9 and SRP14. SRP interacts with ribosomes to bring translated membrane and secreted proteins to the endoplasmic reticulum (ER) for proper processing. SRP9 and SRP14 form a heterodimer before binding to SRP RNA, and SRP19 functions in the assembly of SRP and binds to free SRP RNA. This event is a prerequisite for the subsequent binding of SRP54 to helix 8 of SRP RNA in eukaryotes and involves an SRP19-induced conformational change in the RNA. SRP54 interacts with both the nascent signal peptide and SRP RNA. SRP68 binding to SRP RNA enhances SRP72 binding. SRP19, SRP68 and SRP72 are localized in the nucleolus and cytoplasm, whereas SRP54 is only localized in the cytoplasm. SRP68 also accumulates in the ER. Thus, the nucleolus is the site of assembly and/or interaction between the family of ribonucleoproteins involved in protein synthesis.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using SRP68 (4-YD13): sc-135569 for immunofluorescence, what dilution of this antibody should I use?
Asked by: Trav11
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here:
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn