SRp20 Antibody (7B4): sc-13510
- SRp20 Antibody (7B4) is a mouse monoclonal IgG1 SRp20 antibody, cited in 24 publications, provided at 200 µg/ml
- epitope mapping within amino acids 84-104 of SRp20 of human origin
- SRp20 Antibody (7B4) is recommended for detection of SRp20 of mouse, rat, human and avian origin by WB, IP and IF
- Anti-SRp20 Antibody (7B4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for SRp20 Antibody (7B4). This work is in progress.
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SRp20 Antibody (7B4) is a mouse monoclonal IgG1 antibody that detects SRp20 of mouse, rat, human, and avian origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). SRp20 Antibody (7B4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. SRp20 plays a crucial role in the regulation of pre-mRNA splicing, a vital process for gene expression and the generation of diverse protein isoforms. SRp20 is primarily located in the nucleus, where SRp20 binds to splicing enhancer elements, which are short RNA sequences that activate weak splice sites in nearby introns. This interaction is essential for accurate splice site recognition and the control of alternative splicing, allowing for the production of multiple mRNA variants from a single gene. SRp20, along with other serine/arginine-rich splicing factors, contains RNA recognition motifs and an arginine/serine-rich domain, which are critical for function. Additionally, SRp20 shuttles between the nucleus and cytoplasm, contributing to mRNA transport, while SRp20 activity can be modulated by antagonistic proteins from the heterogeneous nuclear ribonucleoprotein (hnRNP) family, which can shift splicing site selection. The versatility of SRp20 in splicing regulation underscores importance in cellular processes and highlights the relevance of SRp20 (7B4) monoclonal antibody in research focused on gene expression and RNA processing.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SRp20 Antibody (7B4) References:
- The splicing factor-associated protein, p32, regulates RNA splicing by inhibiting ASF/SF2 RNA binding and phosphorylation. | Petersen-Mahrt, SK., et al. 1999. EMBO J. 18: 1014-24. PMID: 10022843
- Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences. | Schaal, TD. and Maniatis, T. 1999. Mol Cell Biol. 19: 1705-19. PMID: 10022858
- The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers. | Cavaloc, Y., et al. 1999. RNA. 5: 468-83. PMID: 10094314
- Global analysis reveals SRp20- and SRp75-specific mRNPs in cycling and neural cells. | Ankö, ML., et al. 2010. Nat Struct Mol Biol. 17: 962-70. PMID: 20639886
- SRp20 is a proto-oncogene critical for cell proliferation and tumor induction and maintenance. | Jia, R., et al. 2010. Int J Biol Sci. 6: 806-26. PMID: 21179588
- Regulation of cellular RNA nano-particle assembly by splicing factor SRp20. | Yoon, SP., et al. 2013. J Nanosci Nanotechnol. 13: 184-7. PMID: 23646715
- SRp20: an overview of its role in human diseases. | Corbo, C., et al. 2013. Biochem Biophys Res Commun. 436: 1-5. PMID: 23685143
- SRp20: A potential therapeutic target for human tumors. | Wang, H. and Jiang, Y. 2021. Pathol Res Pract. 224: 153444. PMID: 34126370
- Function of conserved domains of hnRNP A1 and other hnRNP A/B proteins. | Mayeda, A., et al. 1994. EMBO J. 13: 5483-95. PMID: 7957114
- Specific commitment of different pre-mRNAs to splicing by single SR proteins. | Fu, XD. 1993. Nature. 365: 82-5. PMID: 8361546
- The splicing factor SRp20 modifies splicing of its own mRNA and ASF/SF2 antagonizes this regulation. | Jumaa, H. and Nielsen, PJ. 1997. EMBO J. 16: 5077-85. PMID: 9305649
- A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm. | Cáceres, JF., et al. 1998. Genes Dev. 12: 55-66. PMID: 9420331
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SRp20 Antibody (7B4) | sc-13510 | 200 µg/ml | $322.00 | |||
SRp20 Antibody (7B4) AC | sc-13510 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SRp20 Antibody (7B4) HRP | sc-13510 HRP | 200 µg/ml | $322.00 | |||
SRp20 Antibody (7B4) FITC | sc-13510 FITC | 200 µg/ml | $336.00 | |||
SRp20 Antibody (7B4) PE | sc-13510 PE | 200 µg/ml | $349.00 | |||
SRp20 Antibody (7B4) Alexa Fluor® 488 | sc-13510 AF488 | 200 µg/ml | $364.00 | |||
SRp20 Antibody (7B4) Alexa Fluor® 546 | sc-13510 AF546 | 200 µg/ml | $364.00 | |||
SRp20 Antibody (7B4) Alexa Fluor® 594 | sc-13510 AF594 | 200 µg/ml | $364.00 | |||
SRp20 Antibody (7B4) Alexa Fluor® 647 | sc-13510 AF647 | 200 µg/ml | $364.00 | |||
SRp20 Antibody (7B4) Alexa Fluor® 680 | sc-13510 AF680 | 200 µg/ml | $364.00 | |||
SRp20 Antibody (7B4) Alexa Fluor® 790 | sc-13510 AF790 | 200 µg/ml | $364.00 |