SRA Antibody (D-8): sc-271948

SRA Antibody (D-8) is a mouse monoclonal IgM antibody that detects steroid receptor RNA activator (SRA) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SRA plays a crucial role in mediating the transactivation of steroid hormone receptors, functioning as both an RNA transcript and a stably expressed protein that forms complexes with steroid receptor coactivator-1. SRA contains six essential RNA motifs that are vital for coactivation capabilities, underscoring its importance in regulating gene expression in response to steroid hormones. SRA is widely expressed across various normal tissues, with particularly high levels found in the liver and skeletal muscle, while exhibiting lower expression in the brain. SRA levels are elevated in breast tumors compared to normal tissue, suggesting a potential role in cancer biology. Overexpression of SRA enhances estrogen receptor alpha transcriptional activity, and when cells are transfected with antisense oligodeoxynucleotides targeting SRA, a dose-dependent reduction in estrogen receptor alpha expression occurs. SMRT/HDAC1 associated repressor protein (SHARP) interacts with SRA, inhibiting SRA′s ability to potentiate steroid receptor transcription, further highlighting the complex regulatory mechanisms involved.

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SRA Antibody (D-8) References:

1. A novel steroid receptor co-activator protein (SRAP) as an alternative form of steroid receptor RNA-activator gene: expression in prostate cancer cells and enhancement of androgen receptor activity.  |  Kawashima, H., et al. 2003. Biochem J. 369: 163-71. PMID: 12350225
2. Distinct RNA motifs are important for coactivation of steroid hormone receptors by steroid receptor RNA activator (SRA).  |  Lanz, RB., et al. 2002. Proc Natl Acad Sci U S A. 99: 16081-6. PMID: 12444263
3. The steroid receptor RNA activator is the first functional RNA encoding a protein.  |  Chooniedass-Kothari, S., et al. 2004. FEBS Lett. 566: 43-7. PMID: 15147866
4. Dax-1 and steroid receptor RNA activator (SRA) function as transcriptional coactivators for steroidogenic factor 1 in steroidogenesis.  |  Xu, B., et al. 2009. Mol Cell Biol. 29: 1719-34. PMID: 19188450
5. Increasing the relative expression of endogenous non-coding Steroid Receptor RNA Activator (SRA) in human breast cancer cells using modified oligonucleotides.  |  Cooper, C., et al. 2009. Nucleic Acids Res. 37: 4518-31. PMID: 19483093
6. Mediation of CTCF transcriptional insulation by DEAD-box RNA-binding protein p68 and steroid receptor RNA activator SRA.  |  Yao, H., et al. 2010. Genes Dev. 24: 2543-55. PMID: 20966046
7. Structural architecture of the human long non-coding RNA, steroid receptor RNA activator.  |  Novikova, IV., et al. 2012. Nucleic Acids Res. 40: 5034-51. PMID: 22362738
8. Expression of both estrogen receptor-beta 1 (ER-β1) and its co-regulator steroid receptor RNA activator protein (SRAP) are predictive for benefit from tamoxifen therapy in patients with estrogen receptor-alpha (ER-α)-negative early breast cancer (EBC).  |  Yan, Y., et al. 2013. Ann Oncol. 24: 1986-93. PMID: 23579816
9. Steroid receptor RNA activator protein (SRAP) expression as a prognostic factor in ER+ human breast tumors.  |  Yan, Y., et al. 2013. J Cancer Res Clin Oncol. 139: 1637-47. PMID: 23907597
10. Genome-Wide Transcriptional Regulation of the Long Non-coding RNA Steroid Receptor RNA Activator in Human Erythroblasts.  |  Sawaengdee, W., et al. 2020. Front Genet. 11: 850. PMID: 32849830

The preferred SRA antibody is SRA Antibody (E-5): sc-393240.