SR140 Antibody (E-3): sc-398718

SR140 Antibody (E-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects SR140 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SR140 (E-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SR140, also known as U2-associated SR140 protein or 140 kDa Ser/Arg-rich domain protein, plays a crucial role in pre-mRNA splicing by acting as a splicing enhancer. SR140 contains specific binding sites for serine/arginine-rich splicing factors, which are essential for accurate recognition of splice sites and regulation of alternative splicing. SR140 is composed of 1,029 amino acids and features a CID domain, an RNA recognition motif (RRM) domain, and a SURP motif repeat, highlighting a complex structure vital for function. SR140 is expressed as three alternatively spliced isoforms and is encoded by a gene located on human chromosome 3, underscoring importance in gene expression regulation.

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SR140 Antibody (E-3) References:

1. The splicing factor-associated protein, p32, regulates RNA splicing by inhibiting ASF/SF2 RNA binding and phosphorylation.  |  Petersen-Mahrt, SK., et al. 1999. EMBO J. 18: 1014-24. PMID: 10022843
2. Selection and characterization of pre-mRNA splicing enhancers: identification of novel SR protein-specific enhancer sequences.  |  Schaal, TD. and Maniatis, T. 1999. Mol Cell Biol. 19: 1705-19. PMID: 10022858
3. The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers.  |  Cavaloc, Y., et al. 1999. RNA. 5: 468-83. PMID: 10094314
4. Exonic splicing enhancers: mechanism of action, diversity and role in human genetic diseases.  |  Blencowe, BJ. 2000. Trends Biochem Sci. 25: 106-10. PMID: 10694877
5. Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein.  |  Will, CL., et al. 2002. EMBO J. 21: 4978-88. PMID: 12234937
6. Function of conserved domains of hnRNP A1 and other hnRNP A/B proteins.  |  Mayeda, A., et al. 1994. EMBO J. 13: 5483-95. PMID: 7957114
7. Specific commitment of different pre-mRNAs to splicing by single SR proteins.  |  Fu, XD. 1993. Nature. 365: 82-5. PMID: 8361546
8. The splicing factor SRp20 modifies splicing of its own mRNA and ASF/SF2 antagonizes this regulation.  |  Jumaa, H. and Nielsen, PJ. 1997. EMBO J. 16: 5077-85. PMID: 9305649
9. A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm.  |  Cáceres, JF., et al. 1998. Genes Dev. 12: 55-66. PMID: 9420331

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SR140 Antibody (E-3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-SR140 Antibody (E-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SR140 Antibody (E-3) Specifications

Alternate Product Name: SR140

Product Clone Name: E-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 421-720

Epitope Target: SR140

Epitope Species: human

Product Reactivity/Detection: SR140

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for SR140 Antibody (E-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin