SR-A Antibody (E-5): sc-166184

SR-A Antibody (E-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects SR-A protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). SR-A (E-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The scavenger receptor class A (SR-A) plays a crucial role in lipid metabolism by mediating the uptake of modified low-density lipoprotein (LDL), which is significant for understanding atherosclerosis and other lipid-related diseases. The gene encoding human SR-A is located on chromosome 8 and produces two main isoforms, SR-AI and SR-AII, through alternative splicing. These isoforms consist of six distinct domains: a cytoplasmic domain, a membrane-spanning domain, a spacer domain, an alpha-helical coiled-coil domain, a collagen-like domain, and a type-specific C-terminal domain. The alpha-helical coiled-coil domain enables SR-A trimerization, while the collagen-like domain recognizes diverse ligands. SR-A expression occurs predominantly in the liver, placenta, and brain, with both isoforms facilitating LDL uptake in atherosclerotic lesions. A third isoform, SR-AIII, does not facilitate LDL uptake and may serve as a dominant negative isoform, potentially providing a protective mechanism for cells in advanced atherosclerotic lesions. Beyond lipid metabolism, SR-A contributes to various macrophage-associated pathological processes, including atherosclerosis and Alzheimer′s disease, as well as host defense and cellular adhesion.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SR-A Antibody (E-5) References:

1. Analysis of macrophage scavenger receptor (SR-A) expression in human aortic atherosclerotic lesions.  |  Gough, PJ., et al. 1999. Arterioscler Thromb Vasc Biol. 19: 461-71. PMID: 10073945
2. Class A scavenger receptor up-regulation in smooth muscle cells by oxidized low density lipoprotein. Enhancement by calcium flux and concurrent cyclooxygenase-2 up-regulation.  |  Mietus-Snyder, M., et al. 2000. J Biol Chem. 275: 17661-70. PMID: 10837497
3. Rabbit atherosclerotic lesions express scavenger receptor AIII mRNA, a naturally occurring splice variant that encodes a non-functional, dominant negative form of the macrophage scavenger receptor.  |  Hiltunen, TP., et al. 2001. Atherosclerosis. 154: 415-9. PMID: 11166774
4. The class A scavenger receptor binds to proteoglycans and mediates adhesion of macrophages to the extracellular matrix.  |  Santiago-García, J., et al. 2003. J Biol Chem. 278: 6942-6. PMID: 12488451
5. A role for class A scavenger receptor in dendritic cell nibbling from live cells.  |  Harshyne, LA., et al. 2003. J Immunol. 170: 2302-9. PMID: 12594251
6. Class A scavenger receptor-mediated cell adhesion requires the sequential activation of Lyn and PI3-kinase.  |  Nikolic, DM., et al. 2007. Am J Physiol Cell Physiol. 292: C1450-8. PMID: 17192284
7. Class A scavenger receptor promotes osteoclast differentiation via the enhanced expression of receptor activator of NF-kappaB (RANK).  |  Takemura, K., et al. 2010. Biochem Biophys Res Commun. 391: 1675-80. PMID: 20036645
8. Increased class A scavenger receptor and glomerular lipid precede mesangial matrix expansion in the bGH mouse model.  |  Doi, SQ., et al. 2010. Growth Horm IGF Res. 20: 326-32. PMID: 20576456
9. Human macrophage scavenger receptors: primary structure, expression, and localization in atherosclerotic lesions.  |  Matsumoto, A., et al. 1990. Proc Natl Acad Sci U S A. 87: 9133-7. PMID: 2251254
10. Multiple function of macrophage scavenger receptors mediated by fibrous coiled coil domains.  |  Liao, HS., et al. 1996. Gerontology. 42 Suppl 1: 37-47. PMID: 8964521
11. Scavenger receptors mediate adhesion of activated B lymphocytes.  |  Yokota, T., et al. 1998. Exp Cell Res. 239: 16-22. PMID: 9511720
12. A naturally occurring isoform of the human macrophage scavenger receptor (SR-A) gene generated by alternative splicing blocks modified LDL uptake.  |  Gough, PJ., et al. 1998. J Lipid Res. 39: 531-43. PMID: 9548586

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SR-A Antibody (E-5) has 15 citations in a variety of scientific publications.

Primary antibodies like Anti-SR-A Antibody (E-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SR-A Antibody (E-5) Specifications

Alternate Product Name: CD204

Product Clone Name: E-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 61-250

Epitope Target: SR-A

Epitope Species: human

Product Reactivity/Detection: SR-A

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 15 citations in various scientific publications

Available Conjugates/Forms for SR-A Antibody (E-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin