SPNS2 Double Nickase Plasmid (h): sc-405481-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SPNS2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SPNS2 Double Nickase Plasmid (h) and SPNS2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SPNS2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPNS2 Double Nickase Plasmid (h) | sc-405481-NIC | 20 µg | $410.00 | |||
SPNS2 Double Nickase Plasmid (h2) | sc-405481-NIC-2 | 20 µg | $410.00 |
SPNS2 (spinster homolog 2) encodes a multipass membrane transporter that mediates sphingosine-1-phosphate (S1P) export, shaping extracellular S1P gradients that regulate cell migration, vascular integrity, and immune cell trafficking. By controlling S1P availability for S1PR signaling, SPNS2 interfaces with sphingolipid metabolism and downstream pathways that influence endothelial barrier function and lymphocyte egress. Altered SPNS2 activity has been linked to dysregulated inflammatory responses and vascular phenotypes, and its role in lipid-mediated signaling makes it relevant to studies of tumor microenvironment dynamics and tissue homeostasis. These properties position SPNS2 as a useful target for mechanistic interrogation of lipid transport, receptor-mediated signaling, and cell–cell communication.
SPNS2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SPNS2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SPNS2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SPNS2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SPNS2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.