splicing factor 1 Antibody (H-9): sc-398881

Splicing factor 1 Antibody (H-9) is a mouse monoclonal IgM antibody that detects splicing factor 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-splicing factor 1 antibody (H-9) is available as the non-conjugated form. Splicing factor 1, also known as SF1, zinc finger protein 162, ZFM1, CW17R, and mammalian branch point binding protein (mBBP), plays a crucial role in the splicing process by specifically recognizing the seven-nucleotide branch point sequence located at 3′ splice sites, which is essential for the assembly of early spliceosomal complexes. This function is vital for accurate processing of pre-mRNA, influencing gene expression and cellular function. Splicing factor 1 acts as a transcriptional repressor, potentially regulating proliferation and expression of pro-inflammatory gene products in smooth muscle cells. Expression of splicing factor 1 can be downregulated by cytokines, which may contribute to pathogenesis of hyperproliferative inflammatory diseases. Splicing factor 1 includes a nuclear transport domain, a metal-binding motif, and regions rich in glutamine and proline, which are important for functional interactions. Human splicing factor 1 exists in several isoforms, such as H1-isoform and Bo-isoform, generated through alternative splicing events, adding another layer of complexity to regulatory roles. The gene encoding splicing factor 1 is located on chromosome 11, in proximity to the MEN1 gene, which is associated with multiple endocrine neoplasia-type 1, highlighting potential implications in both normal physiology and disease states.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

splicing factor 1 Antibody (H-9) References:

1. Recognition of RNA branch point sequences by the KH domain of splicing factor 1 (mammalian branch point binding protein) in a splicing factor complex.  |  Peled-Zehavi, H., et al. 2001. Mol Cell Biol. 21: 5232-41. PMID: 11438677
2. Structural basis for recognition of the intron branch site RNA by splicing factor 1.  |  Liu, Z., et al. 2001. Science. 294: 1098-102. PMID: 11691992
3. Cytokine-induced down-regulation of zfm1/splicing factor-1 promotes smooth muscle cell proliferation.  |  Cattaruzza, M., et al. 2002. J Biol Chem. 277: 6582-9. PMID: 11748220
4. Isolation and characterization of a novel gene encoding nuclear protein at a locus (D11S636) tightly linked to multiple endocrine neoplasia type 1 (MEN1).  |  Toda, T., et al. 1994. Hum Mol Genet. 3: 465-70. PMID: 7912130
5. Diverse modes of alternative splicing of human splicing factor SF1 deduced from the exon-intron structure of the gene.  |  Krämer, A., et al. 1998. Gene. 211: 29-37. PMID: 9573336

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-splicing factor 1 Antibody (H-9) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-splicing factor 1 Antibody (H-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

splicing factor 1 Antibody (H-9) Specifications

Alternate Product Name: splicing factor 1

Product Clone Name: H-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 2-23

Epitope Target: splicing factor 1

Epitope Species: human

Product Reactivity/Detection: splicing factor 1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for splicing factor 1 Antibody (H-9): P: Blocking Peptide