SPF30 Antibody (G-8): sc-376887
- SPF30 Antibody (G-8) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 211-238 at the C-terminus of SPF30 of human origin
- SPF30 Antibody (G-8) is recommended for detection of SPF30 of mouse, rat, human and avian origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine, porcine and avian
- Anti-SPF30 Antibody (G-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for SPF30 Antibody (G-8) for WB applications. This reagent is now offered in a bundle with SPF30 Antibody (G-8) (see ordering information below).
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SPF30 Antibody (G-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects SPF30 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). SPF30 (G-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SPF30, also known as survival of motor neuron-related splicing factor 30 or SMNDC1, is a crucial splicing factor that plays a significant role in the assembly of the spliceosome, a complex essential for mRNA processing. SPF30 is primarily localized in the nucleus, specifically within Cajal bodies and nuclear speckles, which are important sites for RNA processing and assembly. Proper localization of SPF30 is vital for facilitating the recruitment of U4/U5/U6 tri-snRNPs to the prespliceosome, thereby ensuring efficient spliceosome assembly. Disruption of SPF30 function can lead to severe consequences, including the arrest of spliceosome assembly and potential cellular apoptosis, highlighting SPF30′s importance in maintaining cellular homeostasis and proper gene expression.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
SPF30 Antibody (G-8) References:
- SPF30 is an essential human splicing factor required for assembly of the U4/U5/U6 tri-small nuclear ribonucleoprotein into the spliceosome. | Rappsilber, J., et al. 2001. J Biol Chem. 276: 31142-50. PMID: 11331295
- SMNrp is an essential pre-mRNA splicing factor required for the formation of the mature spliceosome. | Meister, G., et al. 2001. EMBO J. 20: 2304-14. PMID: 11331595
- Coupled in vitro import of U snRNPs and SMN, the spinal muscular atrophy protein. | Narayanan, U., et al. 2004. Mol Cell. 16: 223-34. PMID: 15494309
- Tudor domains bind symmetrical dimethylated arginines. | Côté, J. and Richard, S. 2005. J Biol Chem. 280: 28476-83. PMID: 15955813
- Radiation hybrid mapping of the pig SMNDC1, ARPP-19 and PRO2730 genes to SSC14, SSC1 and SSC13 respectively. | Ren, HY., et al. 2006. Cytogenet Genome Res. 112: 341E. PMID: 16484796
- Splicing factor SPF30 bridges an interaction between the prespliceosome protein U2AF35 and tri-small nuclear ribonucleoprotein protein hPrp3. | Little, JT. and Jurica, MS. 2008. J Biol Chem. 283: 8145-52. PMID: 18211889
- Mass spectrometry and EST-database searching allows characterization of the multi-protein spliceosome complex. | Neubauer, G., et al. 1998. Nat Genet. 20: 46-50. PMID: 9731529
- Characterization of a gene encoding survival motor neuron (SMN)-related protein, a constituent of the spliceosome complex. | Talbot, K., et al. 1998. Hum Mol Genet. 7: 2149-56. PMID: 9817934
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPF30 Antibody (G-8) | sc-376887 | 200 µg/ml | $322.00 | |||
SPF30 Antibody (G-8): m-IgG Fc BP-HRP Bundle | sc-526059 | 200 µg Ab; 10 µg BP | $361.00 | |||
SPF30 Antibody (G-8) AC | sc-376887 AC | 500 µg/ml, 25% agarose | $424.00 | |||
SPF30 Antibody (G-8) HRP | sc-376887 HRP | 200 µg/ml | $322.00 | |||
SPF30 Antibody (G-8) FITC | sc-376887 FITC | 200 µg/ml | $336.00 | |||
SPF30 Antibody (G-8) PE | sc-376887 PE | 200 µg/ml | $349.00 | |||
SPF30 Antibody (G-8) Alexa Fluor® 488 | sc-376887 AF488 | 200 µg/ml | $364.00 | |||
SPF30 Antibody (G-8) Alexa Fluor® 546 | sc-376887 AF546 | 200 µg/ml | $364.00 | |||
SPF30 Antibody (G-8) Alexa Fluor® 594 | sc-376887 AF594 | 200 µg/ml | $364.00 | |||
SPF30 Antibody (G-8) Alexa Fluor® 647 | sc-376887 AF647 | 200 µg/ml | $364.00 | |||
SPF30 Antibody (G-8) Alexa Fluor® 680 | sc-376887 AF680 | 200 µg/ml | $364.00 | |||
SPF30 Antibody (G-8) Alexa Fluor® 790 | sc-376887 AF790 | 200 µg/ml | $364.00 | |||
SPF30 (G-8) Neutralizing Peptide | sc-376887 P | 100 µg/0.5 ml | $69.00 |