SPATA18 Double Nickase Plasmid (h): sc-407015-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SPATA18 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SPATA18 Double Nickase Plasmid (h) and SPATA18 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SPATA18. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SPATA18 Double Nickase Plasmid (h) | sc-407015-NIC | 20 µg | $410.00 |
SPATA18 (also known as MIEAP) encodes a stress-responsive protein that supports mitochondrial quality control by promoting lysosome-dependent elimination or repair of damaged mitochondria, helping preserve respiratory function and limit oxidative stress. It is regulated downstream of p53 signaling and is linked to mitochondrial dynamics, mitophagy-related processes, and cellular responses to DNA damage and metabolic stress. Altered SPATA18 activity has been associated with mitochondrial dysfunction phenotypes that are relevant to cancer biology, neurodegeneration, and cardiometabolic research contexts. Studying SPATA18 provides a handle for dissecting how p53-coordinated mitochondrial surveillance influences cell fate decisions and redox homeostasis.
SPATA18 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SPATA18 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SPATA18. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SPATA18 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SPATA18-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.