SPAG5 Antibody (LX-8): sc-100885

SPAG5 Antibody (LX-8) is a mouse monoclonal IgG3 kappa light chain antibody that detects the SPAG5 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-SPAG5 antibody (LX-8) is available as the non-conjugated format. SPAG5, also known as sperm associated antigen 5, MAP126, hMAP126, DEEPEST, or Astrin, is a 1,193 amino acid protein predominantly expressed in the testis, with lower expression levels found in the liver, placenta, pancreas, colon, and thymus. SPAG5 protein is critically localized to spindle poles during prophase and throughout the spindle during metaphase and anaphase, making SPAG5 essential for proper spindle formation. SPAG5 plays a crucial role in the dynamic regulation of mitotic spindles, which is essential for accurate chromosome segregation during cell division. Additionally, SPAG5 is involved in sister chromatid cohesion and the subsequent separation of chromatids, thereby serving as a vital regulator of chromosome integrity. SPAG5 features a long stalk and a globular head domain that allows SPAG5 to form a homodimer characterized by aster-like structures, which are essential for its function. Notably, upon DNA damage, SPAG5 may undergo phosphorylation by ataxia-telangiectasia mutated (ATM) or ataxia telangiectasia and Rad3-related (ATR) kinases, highlighting SPAG5′s involvement in cellular responses to genomic stress.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

SPAG5 Antibody (LX-8) References:

1. Testicular protein Spag5 has similarity to mitotic spindle protein Deepest and binds outer dense fiber protein Odf1.  |  Shao, X., et al. 2001. Mol Reprod Dev. 59: 410-6. PMID: 11468777
2. Cloning and characterization of hMAP126, a new member of mitotic spindle-associated proteins.  |  Chang, MS., et al. 2001. Biochem Biophys Res Commun. 287: 116-21. PMID: 11549262
3. Analysis of mitotic microtubule-associated proteins using mass spectrometry identifies astrin, a spindle-associated protein.  |  Mack, GJ. and Compton, DA. 2001. Proc Natl Acad Sci U S A. 98: 14434-9. PMID: 11724960
4. The mitotic-spindle-associated protein astrin is essential for progression through mitosis.  |  Gruber, J., et al. 2002. J Cell Sci. 115: 4053-9. PMID: 12356910
5. Silencing of astrin induces the p53-dependent apoptosis by suppression of HPV18 E6 expression and sensitizes cells to paclitaxel treatment in HeLa cells.  |  Yang, YC., et al. 2006. Biochem Biophys Res Commun. 343: 428-34. PMID: 16546135
6. Phosphoproteome analysis of the human mitotic spindle.  |  Nousiainen, M., et al. 2006. Proc Natl Acad Sci U S A. 103: 5391-6. PMID: 16565220
7. hNinein is required for targeting spindle-associated protein Astrin to the centrosome during the S and G2 phases.  |  Cheng, TS., et al. 2007. Exp Cell Res. 313: 1710-21. PMID: 17383637
8. Astrin is required for the maintenance of sister chromatid cohesion and centrosome integrity.  |  Thein, KH., et al. 2007. J Cell Biol. 178: 345-54. PMID: 17664331
9. Glycogen synthase kinase 3beta interacts with and phosphorylates the spindle-associated protein astrin.  |  Cheng, TS., et al. 2008. J Biol Chem. 283: 2454-64. PMID: 18055457