SP Antibody (4G7C3): sc-517213

SP Antibody (4G7C3) is a mouse monoclonal IgG1 antibody that detects the SP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-SP antibody (4G7C3) is available as the non-conjugated form. SP protein, also known as pS2, plays a crucial role in mucosal healing and is part of the trefoil factor family, which consists of protease-resistant peptides secreted throughout the gastrointestinal tract. These trefoil peptides are characterized by their unique three-dimensional structure, which includes three intrachain disulfide bonds that form the trefoil motif, or P-domain. This specific structure is essential for SP protein′s stability and function, allowing SP protein to protect and repair the mucosal lining of the gut. pS2 is highly expressed in normal gastric mucosa and has been implicated in various carcinomas, including those of the breast, pancreas, and stomach. Notably, pS2 is absent in normal breast tissue but can be induced by estrogen in MCF7 cells, highlighting pS2′s potential role in cancer biology. Additionally, pS2 forms dimers, a process believed to enhance protective and healing properties, making pS2 a significant target for research in gastrointestinal health and cancer. The gene encoding pS2 is located on human chromosome 21q22.3, further emphasizing pS2′s relevance in human health and disease.

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SP Antibody (4G7C3) References:

1. Two antiestrogens affect differently chromatin remodeling of trefoil factor 1 (pS2) gene and the fate of estrogen receptor in MCF7 cells.  |  Giamarchi, C., et al. 2002. Biochim Biophys Acta. 1578: 12-20. PMID: 12393183
2. Selective abrogation of the proinvasive activity of the trefoil peptides pS2 and spasmolytic polypeptide by disruption of the EGF receptor signaling pathways in kidney and colonic cancer cells.  |  Rodrigues, S., et al. 2003. Oncogene. 22: 4488-97. PMID: 12881705
3. Hemokinins and endokinins.  |  Page, NM. 2004. Cell Mol Life Sci. 61: 1652-63. PMID: 15224188
4. Antibody-based exosite inhibitors of ADAMTS-5 (aggrecanase-2).  |  Santamaria, S., et al. 2015. Biochem J. 471: 391-401. PMID: 26303525
5. Clinical significance of the estrogen regulated pS2 protein in mammary tumors.  |  Pichon, MF. and Milgrom, E. 1993. Crit Rev Oncol Hematol. 15: 13-21. PMID: 8240704
6. Human trefoil peptides: genomic structure in 21q22.3 and coordinated expression.  |  Gött, P., et al. 1996. Eur J Hum Genet. 4: 308-15. PMID: 9043862
7. Trefoil proteins: their role in normal and malignant cells.  |  May, FE. and Westley, BR. 1997. J Pathol. 183: 4-7. PMID: 9370940
8. Intestinal trefoil factor (TFF 3) and pS2 (TFF 1), but not spasmolytic polypeptide (TFF 2) mRNAs are co-expressed in normal, hyperplastic, and neoplastic human breast epithelium.  |  Poulsom, R., et al. 1997. J Pathol. 183: 30-8. PMID: 9370944
9. Trefoil peptides: from structure to function.  |  Thim, L. 1997. Cell Mol Life Sci. 53: 888-903. PMID: 9447240
10. Dimerization of human pS2 (TFF1) plays a key role in its protective/healing effects.  |  Marchbank, T., et al. 1998. J Pathol. 185: 153-8. PMID: 9713341
11. The pS2/TFF1 trefoil factor, from basic research to clinical applications.  |  Ribieras, S., et al. 1998. Biochim Biophys Acta. 1378: F61-77. PMID: 9739760
12. Growth factors and the gastrointestinal tract.  |  Murphy, MS. 1998. Nutrition. 14: 771-4. PMID: 9785359

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SP Antibody (4G7C3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-SP Antibody (4G7C3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SP Antibody (4G7C3) Specifications

Alternate Product Name: SP

Product Clone Name: 4G7C3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 20-125

Epitope Target: SP

Epitope Species: human

Product Reactivity/Detection: SP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for SP Antibody (4G7C3): N/A