Sox-4 CRISPR/Cas9 KO Plasmid (h): sc-401217
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Sox-4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Sox-4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Sox-4 Antibody (B-7): sc-518016
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sox-4 CRISPR/Cas9 KO Plasmid (h) | sc-401217 | 20 µg | $397.00 |
Overview
SOX4 encodes the transcription factor Sox-4, a high-mobility group (HMG) DNA-binding protein that regulates lineage specification, differentiation, and cell fate decisions during development and tissue homeostasis. Sox-4 participates in transcriptional programs linked to Wnt/β-catenin and TGF-β/SMAD signaling, and it can influence epithelial–mesenchymal transition, cell-cycle control, and apoptosis depending on cellular context. Altered SOX4 expression has been associated with dysregulated progenitor maintenance and aberrant transcriptional networks observed across multiple cancer and immunologic research settings. As a nodal regulator of gene expression, Sox-4 is frequently studied for its effects on chromatin-associated transcriptional circuitry and pathway cross-talk.
Sox-4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SOX4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SOX4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SOX4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Sox-4 protein expression.
This CRISPR knockout system enables efficient generation of SOX4-deficient cell models for investigation of Sox-4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SOX4 exon(s) critical for Sox-4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SOX4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Sox-4 CRISPR/Cas9 KO Plasmid (h) and Sox-4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SOX4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Sox-4 HDR Plasmid (h) and Sox-4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SOX4 homology arms to support homology-directed repair at defined SOX4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.