Sol8 Cell Lysate: sc-2249

Sol8 Cell Lysate is derived from Sol8 cells, a murine myoblast cell line originally established from mouse skeletal muscle. This lysate is widely used in muscle biology and differentiation research to investigate the molecular mechanisms underlying myogenesis and muscle regeneration. Sol8 cells have the ability to differentiate into multinucleated myotubes, making them a valuable model for studying muscle cell development and function. Researchers utilize Sol8 Cell Lysate to analyze the expression, phosphorylation, and interactions of key proteins involved in muscle-specific signaling pathways, such as the PI3K/AKT/mTOR and MAPK/ERK pathways, using techniques like western blotting, immunoprecipitation, and mass spectrometry. This lysate is particularly useful for examining the roles of transcription factors like MyoD and Myogenin in muscle differentiation and the impact of various growth factors and cytokines on myogenic signaling. Additionally, Sol8 Cell Lysate aids in exploring the molecular basis of muscle hypertrophy and atrophy by studying the expression of muscle-specific proteins, such as myosin heavy chain and actin. By comparing Sol8 lysates with those from undifferentiated myoblasts or differentiated myotubes, scientists can identify specific molecular alterations associated with muscle development, advancing research in muscle biology, cell differentiation, and regeneration.

Sol8 Cell Lysate References:

1. Astrocytes and microglia differentially regulate trafficking of lymphocyte subsets across brain endothelial cells.  |  Hudson, LC., et al. 2005. Brain Res. 1058: 148-60. PMID: 16137663
2. Ethanol suppression of peripheral blood mononuclear cell trafficking across brain endothelial cells in immunodeficiency virus infection.  |  Hudson, LC., et al. 2010. HIV AIDS (Auckl). 2: 7-18. PMID: 22096380