Sodium phosphate monobasic, anhydrous (CAS 7558-80-7)

Sodium phosphate monobasic, anhydrous, is a vital chemical compound extensively used in various research applications. Its mechanism of action primarily revolves around its role as a buffering agent and a source of phosphate ions in biochemical and molecular biology studies. In research laboratories, it serves as a crucial component in buffer solutions, particularly in the preparation of phosphate-buffered saline (PBS), which is widely utilized in cell culture, protein biochemistry, and molecular biology techniques. Additionally, sodium phosphate monobasic is employed in nucleic acid isolation procedures, where its buffering capacity helps maintain the stability and integrity of DNA and RNA molecules during purification steps. Moreover, this compound plays a pivotal role in the preparation of electrophoresis running buffers for DNA and protein gel electrophoresis, facilitating the separation and analysis of biomolecules based on their size and charge. Beyond its buffering properties, sodium phosphate monobasic is also utilized in the formulation of microbiological growth media to support the growth of bacteria and other microorganisms in research settings. Furthermore, it finds applications in analytical chemistry as a reagent for the determination of phosphate levels in environmental and biological samples through spectrophotometric or colorimetric assays. Overall, sodium phosphate monobasic, anhydrous, serves as a versatile chemical reagent in research laboratories, enabling a wide range of experiments in biochemistry, molecular biology, microbiology, and analytical chemistry.