Sodium phosphate dibasic solution (CAS 7558-79-4)

Sodium phosphate dibasic solution is a highly utilized chemical in scientific research, particularly for its role as a buffering agent in biological and chemical experiments. This solution maintains pH stability crucial for numerous biochemical processes, particularly in molecular biology and biochemistry. Its buffering capacity is essential for maintaining the environment needed for the activity of various enzymes, including those used in DNA replication and transcription processes. In research applications, sodium phosphate dibasic is often used in the preparation of buffer solutions for cell culture systems, where it helps regulate the pH to maintain cellular function and viability during experimental manipulations. Additionally, this compound is instrumental in protein purification techniques. It facilitates the solubilization of proteins and their subsequent separation by affecting the ionic strength of the solution, which is critical during the isolation process. Its use extends to chromatography and electrophoresis, where precise pH control is necessary to achieve accurate molecular separations. Researchers also employ sodium phosphate dibasic in the preparation of reagents for immunohistochemistry, providing an optimal pH for antigen retrieval and staining processes, thus enhancing the clarity and resolution of imaging in structural and cellular studies. This broad utility highlights its integral role in advancing experimental science by ensuring experimental conditions are optimal for various biochemical and cellular analyses.