Sodium Citrate, Dihydrate (CAS 6132-04-3)

Potassium Phosphate, Monobasic is a chemical compound composed of potassium (K+) and dihydrogen phosphate ions (H2PO4^-). This salt is widely used in scientific research primarily for its excellent buffering properties. It plays a crucial role in preparing buffer solutions that maintain a stable pH in biological and chemical systems, typically within the mildly acidic range. The buffering action of potassium phosphate, monobasic, involves its ability to neutralize excess bases by reacting with hydroxide ions to form dibasic potassium phosphate. Similarly, it can adjust to increased acidity by reacting with acids to release hydrogen ions, thus maintaining pH stability. This dual capability is essential for supporting the precise pH conditions necessary for various biochemical processes, including enzymatic reactions where pH stability is critical for maintaining enzyme activity and structural integrity. In the realm of research, it is extensively used in the formulation of phosphate buffers for cell culture media, facilitating essential cellular functions and energy transfers, notably in ATP synthesis. Additionally, it finds significant applications in molecular biology, especially in protocols for DNA and RNA extractions, where a specific and stable pH is vital for preserving the integrity of nucleic acids. This makes potassium phosphate, monobasic an indispensable component in diverse laboratory settings, ensuring controlled and consistent experimental conditions.