SNX10 Double Nickase Plasmid (h): sc-416307-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SNX10 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SNX10 Double Nickase Plasmid (h) and SNX10 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SNX10. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: SNX10 Antibody (1G5): sc-293380
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SNX10 Double Nickase Plasmid (h) | sc-416307-NIC | 20 µg | $410.00 | |||
SNX10 Double Nickase Plasmid (h2) | sc-416307-NIC-2 | 20 µg | $410.00 |
SNX10 (sorting nexin 10) is a PX-domain sorting nexin that binds phosphoinositides and helps coordinate endosomal membrane trafficking, cargo sorting, and organelle dynamics. By coupling lipid recognition to vesicular transport machinery, SNX10 contributes to endosome maturation, receptor turnover, and intracellular signaling homeostasis. SNX10 activity has also been linked to osteoclast function and vesicular acidification processes that influence bone remodeling and cellular degradative pathways. Dysregulation of SNX10-associated trafficking programs is relevant to studies of skeletal biology and disorders involving altered endolysosomal transport.
SNX10 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SNX10 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SNX10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SNX10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SNX10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.