SnoN Antibody (B-3): sc-136958

SnoN Antibody (B-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects SnoN protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-SnoN antibody (B-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. SnoN protein, a member of the Ski family of oncogenes, plays a crucial role in regulating TGFβ signaling pathways, which are essential for controlling cell growth and differentiation. SnoN functions primarily as a transcriptional repressor, inhibiting the activity of Smad proteins, which are key mediators of TGFβ signaling. By binding to the MH2 domain of Smad2 and Smad4, SnoN recruits the transcriptional co-repressor N-CoR, effectively blocking the transcription of target genes that promote cell proliferation and extracellular matrix production. Regulation of SnoN is vital, as TGFβ signaling can lead to degradation, thereby allowing for the activation of Smad-dependent transcription and promoting cellular responses to TGFβ. Understanding the dynamics of SnoN and interactions with Smad proteins is important for elucidating the mechanisms of TGFβ-mediated cellular processes and their implications in various diseases, including cancer.

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SnoN Antibody (B-3) References:

1. Negative feedback regulation of TGF-beta signaling by the SnoN oncoprotein.  |  Stroschein, SL., et al. 1999. Science. 286: 771-4. PMID: 10531062
2. A new blocker for the TGF-beta pathway.  |  Vogel, G. 1999. Science. 286: 665. PMID: 10577218
3. Downregulation of Ski and SnoN co-repressors by anisomycin.  |  Vázquez-Macías, A., et al. 2005. FEBS Lett. 579: 3701-6. PMID: 15967445
4. SKI pathways inducing progression of human melanoma.  |  Reed, JA., et al. 2005. Cancer Metastasis Rev. 24: 265-72. PMID: 15986136
5. Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p.  |  Wang, L., et al. 2005. RNA. 11: 1291-302. PMID: 16043509
6. SnoN suppresses TGF-β-induced epithelial-mesenchymal transition and invasion of bladder cancer in a TIF1γ-dependent manner.  |  Yin, X., et al. 2016. Oncol Rep. 36: 1535-41. PMID: 27430247
7. Isolation of human cDNA clones of ski and the ski-related gene, sno.  |  Nomura, N., et al. 1989. Nucleic Acids Res. 17: 5489-500. PMID: 2762147
8. BMP-7 ameliorates partial epithelial-mesenchymal transition by restoring SnoN protein level via Smad1/5 pathway in diabetic kidney disease.  |  Peng, W., et al. 2022. Cell Death Dis. 13: 254. PMID: 35314669
9. A carboxyl-terminal region of the ski oncoprotein mediates homodimerization as well as heterodimerization with the related protein SnoN.  |  Heyman, HC. and Stavnezer, E. 1994. J Biol Chem. 269: 26996-7003. PMID: 7929440
10. SnoI, a novel alternatively spliced isoform of the ski protooncogene homolog, sno.  |  Pearson-White, S. 1993. Nucleic Acids Res. 21: 4632-8. PMID: 8233802
11. Complex formation between proteins encoded by the ski gene family.  |  Nagase, T., et al. 1993. J Biol Chem. 268: 13710-6. PMID: 8514802
12. A transient increase of snoN transcript by growth arrest upon serum deprivation and cell-to-cell contact.  |  Mimura, N., et al. 1996. FEBS Lett. 397: 253-9. PMID: 8955358

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-SnoN Antibody (B-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-SnoN Antibody (B-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

SnoN Antibody (B-3) Specifications

Alternate Product Name: Ski-Related Oncogene SnoN

Product Clone Name: B-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 368-684

Epitope Target: SnoN

Epitope Species: human

Product Reactivity/Detection: SnoN and SnoN2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for SnoN Antibody (B-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent