Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Snapin CRISPR/Cas9 KO Plasmid (h): sc-406317
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Snapin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Snapin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Snapin Antibody (E-10): sc-514675
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Snapin CRISPR/Cas9 KO Plasmid (h) | sc-406317 | 20 µg | $397.00 |
Overview
SNAPIN encodes Snapin, a small cytosolic protein that associates with SNARE machinery and tethering complexes to support vesicle docking and regulated exocytosis, particularly in neurons and neuroendocrine cells. Snapin has been linked to synaptic vesicle priming, calcium-coupled neurotransmitter release, and endosome/lysosome-related trafficking through interactions that coordinate membrane fusion events. By influencing synaptic transmission and intracellular transport, SNAPIN is frequently studied in pathways relevant to neuronal connectivity, autophagy-lysosome function, and stress-responsive signaling. Altered Snapin-dependent trafficking has been investigated in the context of neurodevelopmental and neurodegenerative disease mechanisms where vesicle transport and proteostasis are disrupted.
Snapin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SNAPIN gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SNAPIN together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SNAPIN open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Snapin protein expression.
This CRISPR knockout system enables efficient generation of SNAPIN-deficient cell models for investigation of Snapin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SNAPIN exon(s) critical for Snapin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SNAPIN genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Snapin CRISPR/Cas9 KO Plasmid (h) and Snapin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SNAPIN locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Snapin HDR Plasmid (h) and Snapin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SNAPIN homology arms to support homology-directed repair at defined SNAPIN target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.