Smallpox BR5 Antibody (1J2F3): sc-52907

Smallpox BR5 Antibody (1J2F3) is a mouse monoclonal IgG1 antibody that detects the Smallpox BR5 protein of viral origin by western blotting (WB). Anti-Smallpox BR5 antibody (1J2F3) is available as a non-conjugated format, specifically targeting the B5R protein, which is a type I membrane glycoprotein crucial for the formation of extracellular enveloped virus (EEV). B5R protein plays a significant role in the viral life cycle, as B5R is essential for the efficient wrapping of intracellular mature virus (IMV), actin tail formation, and the overall virulence of the virus. B5R′s large ectodomain, transmembrane region, and cytoplasmic tail contribute to functionality, allowing for proper incorporation into EEV and facilitating cell-to-cell spread of the virus. Understanding B5R protein function is vital, as B5R not only aids in the development of effective vaccines but also provides insights into potential therapeutic targets for controlling smallpox and related viral infections.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Smallpox BR5 Antibody (1J2F3) References:

1. An investigation of incorporation of cellular antigens into vaccinia virus particles.  |  Krauss, O., et al. 2002. J Gen Virol. 83: 2347-2359. PMID: 12237415
2. Pathologic findings in hemorrhagic smallpox (purpura variolosa); report of case, with special reference to Feulgen's reaction in tissue.  |  WOLMAN, M. 1951. Am J Clin Pathol. 21: 1127-38. PMID: 14885136
3. Antibodies against the extracellular enveloped virus B5R protein are mainly responsible for the EEV neutralizing capacity of vaccinia immune globulin.  |  Bell, E., et al. 2004. Virology. 325: 425-31. PMID: 15246280
4. Papular spongiotic dermatitis of smallpox vaccination: report of 2 cases with review of the literature.  |  Gaertner, EM., et al. 2004. Arch Pathol Lab Med. 128: 1173-5. PMID: 15387700
5. Case of confluent smallpox treated with penicillin and streptomycin.  |  MURPHY, E. 1950. Nurs Mirror Midwives J. 91: 81-2. PMID: 15417055
6. Terminal region sequence variations in variola virus DNA.  |  Massung, RF., et al. 1996. Virology. 221: 291-300. PMID: 8661439
7. The extracellular domain of vaccinia virus protein B5R affects plaque phenotype, extracellular enveloped virus release, and intracellular actin tail formation.  |  Mathew, E., et al. 1998. J Virol. 72: 2429-38. PMID: 9499104
8. Roles of vaccinia virus EEV-specific proteins in intracellular actin tail formation and low pH-induced cell-cell fusion.  |  Sanderson, CM., et al. 1998. J Gen Virol. 79 (Pt 6): 1415-25. PMID: 9634084
9. Functional analysis of vaccinia virus B5R protein: role of the cytoplasmic tail.  |  Lorenzo, MM., et al. 1998. Virology. 252: 450-7. PMID: 9878625